睡眠剥夺模型小鼠尿液的蛋白质组和代谢组分析

doi:10.16352/j.issn.1001-6325.2026.05.0614

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (5): 614-620.doi: 10.16352/j.issn.1001-6325.2026.05.0614

睡眠剥夺模型小鼠尿液的蛋白质组和代谢组分析

张玉雪¹, 刘婷婷¹, 何梦真¹, 胡斯奇², 李军², 孙伟^1*

1.中国医学科学院北京协和医学院基础医学研究所药理学系, 北京 100005;
2.中国人民解放军总医院第七医学中心,儿科医学部研究所北京 100010

收稿日期:2025-12-17 修回日期:2026-03-24 出版日期:2026-05-05 发布日期:2026-04-28
通讯作者: ^*sunwei@ibms.pumc.edu.cn
基金资助:
国家自然科学基金(82170524,31901039)

Proteomic and metabolomic analysis of urine in sleep-deprived mouse models

ZHANG Yuxue¹, LIU Tingting¹, HE Mengzhen¹, HU Siqi², LI Jun², SUN Wei^1*

1. Department of Pharmacology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005;
2. Institute of Pediatrics, Senior Department of Pediatrics, the Seventh Medical Center of Chinese PLA General Hospital, Beijing 100010, China

Received:2025-12-17 Revised:2026-03-24 Online:2026-05-05 Published:2026-04-28
Contact: ^*sunwei@ibms.pumc.edu.cn

摘要/Abstract

摘要： 目的通过分析睡眠剥夺(SD)模型小鼠的尿液蛋白质组和代谢组,揭示睡眠剥夺的病理生理机制。方法 12只雄性小鼠随机分为对照组和模型组,每组6只,连续8 d每天从代谢笼中收集尿液,用于液质联用(LC-MS)蛋白质组和代谢组分析。结果从睡眠剥夺模型小鼠尿液中鉴定到855个差异蛋白质的功能注释分析,通路集中在补体和凝血级联反应以及谷胱甘肽代谢通路。鉴定到158个差异代谢物通路集中在色素代谢过程、信号转导与调控、免疫反应激活等代谢通路中。蛋白质和代谢物联合分析表明,功能主要集中在谷胱甘肽代谢、补体和凝血级联反应通路。结论睡眠剥夺相关蛋白质和代谢物代谢通路的改变,为了解睡眠剥夺的机制提供了新的线索。

关键词: 睡眠剥夺, 蛋白质组学, 代谢组学, 尿液

Abstract: Objective To explore the patho-physiological changes of sleep deprivation (SD) by analyzing the urinary proteome and metabolome of sleep-deprived mouse models. Methods Twelve male mice were randomly divided into a control group and a model group with six in each. Urine samples were collected daily from metabolic cages for 8 consecutive days and subjected to liquid chromatography-mass spectrometry (LC-MS)-based proteomic and metabolomic analyses. Results A total of 855 differential proteins were identified in the urine of sleep-deprived mouse models. Function annotation analysis showed an enrichment in the complement and coagulation cascades and glutathione metabolism pathways. Additionally, 158 differential metabolites were identified, which were primarily enriched in metabolic pathways such as pigment metabolic processes, signal transduction/ regulation and immune response activation. Integrated proteomic and metabolomic analysis revealed that the core function pathways were glutathione metabolism and complement and coagulation cascades. Conclusions Changes in SD-related protein and metabolite metabolic pathways provide novel insights into the mechanisms underlying sleep deprivation.

Key words: sleep deprivation, proteomics, metabolomics, urine

中图分类号:

R388.63

张玉雪, 刘婷婷, 何梦真, 胡斯奇, 李军, 孙伟. 睡眠剥夺模型小鼠尿液的蛋白质组和代谢组分析[J]. 基础医学与临床, 2026, 46(5): 614-620.

ZHANG Yuxue, LIU Tingting, HE Mengzhen, HU Siqi, LI Jun, SUN Wei. Proteomic and metabolomic analysis of urine in sleep-deprived mouse models[J]. Basic & Clinical Medicine, 2026, 46(5): 614-620.

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睡眠剥夺模型小鼠尿液的蛋白质组和代谢组分析

Proteomic and metabolomic analysis of urine in sleep-deprived mouse models

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