成人H3K27-变异型弥漫性中线胶质瘤蛋白质组特征分析

doi:10.16352/j.issn.1001-6325.2026.05.0607

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (5): 607-613.doi: 10.16352/j.issn.1001-6325.2026.05.0607

成人H3K27-变异型弥漫性中线胶质瘤蛋白质组特征分析

倪艳颖¹, 翟春颜¹, 沈萍¹, 张繁霜², 姜忠彩^1*

1.航空总医院病理科,北京 100012;
2.国家癌症中心/国家肿瘤临床研究中心/中国医学科学院北京协和医学院肿瘤医院病理科,北京 100021

收稿日期:2025-09-24 修回日期:2025-12-26 出版日期:2026-05-05 发布日期:2026-04-28
通讯作者: ^*757502492@qq.com
基金资助:
国家自然科学基金(82403823)

Proteomic characteristic analysis of adult H3K27-altered diffuse midline glioma

NI Yanying¹, ZHAI Chunyan¹, SHEN Ping¹, ZHANG Fanshuang², JIANG Zhongcai^1*

1. Department of Pathology, Aviation General Hospital, Beijing 100012;
2. Department of Pathology, National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021, China

Received:2025-09-24 Revised:2025-12-26 Online:2026-05-05 Published:2026-04-28
Contact: ^*757502492@qq.com

摘要/Abstract

摘要： 目的分析成人 H3K27-变异型弥漫性中线胶质瘤(DMG)的临床病理学资料,结合肿瘤组织的蛋白质差异表达信息,探讨成人H3K27-变异型DMG的特征,为疾病提供更加全面的诊断线索。方法收集8例H3K27-变异型DMG成人患者的临床病理资料,使用激光显微切割结合质谱技术对肿瘤组织标本进行蛋白质组学分析。将筛选出的差异表达蛋白质进行功能分析,并选择其中重要的差异表达蛋白质进行免疫组织化学验证。结果此次组织样本共鉴定到 6 171 个蛋白质。与对照组相比,在肿瘤组中鉴定到 867个差异表达蛋白质;与瘤旁组相比,肿瘤组中鉴定到277个差异蛋白质。主要与mRNA和蛋白质合成有关。其中在肿瘤组织中高表达的CTBP2、YBX1、SRSF1和SRSF2得到了免疫组化的验证(P＜0.05)。结论成人H3K27-变异型 DMG的肿瘤组织具有显著的蛋白质组特征差异,可以为诊断提供更全面的信息。

关键词: H3K27-变异型弥漫性中线胶质瘤, 成人, 临床病理特征, 差异蛋白质

Abstract: Objective To investigate pathological characteristics of adult H3K27-altered diffuse midline glioma (DMG) and differential protein expression in tumor brain tissue, for providing more comprehensive diagnostic clues. Methods Clinical and pathological data from eight adult patients with H3K27-altered DMG were collected. Proteomics analysis of tumor tissue was performed using laser microdissection combined with mass spectrometry. Differentially expressed proteins were screened for functional analysis, and four key proteins among these proteins were selected for immuno-histochemical validation. Results A total of 6 171 proteins were identified in these tissue samples. Compared with the non-tumor control group, 867 proteins were significantly expressed in the tumor group; compared with the para-neoplastic group, 277 proteins were significantly expressed tumor group. It was mainly related to mRNA synthesis and protein synthesis. CTBP2, YBX1, SRSF1, and SRSF2, which were significantly up-regulated in tumor tissues, were verified by immuno-histochemistry(P＜0.05). Conclusions The tumor tissue exhibits significant proteomic characteristic differences. Differential protein expression analysis can provide more comprehensive information to support diagnosis.

Key words: H3K27-altered diffuse midline glioma, adult, pathological characteristic, differential proteins

中图分类号:

R446.8

倪艳颖, 翟春颜, 沈萍, 张繁霜, 姜忠彩. 成人H3K27-变异型弥漫性中线胶质瘤蛋白质组特征分析[J]. 基础医学与临床, 2026, 46(5): 607-613.

NI Yanying, ZHAI Chunyan, SHEN Ping, ZHANG Fanshuang, JIANG Zhongcai. Proteomic characteristic analysis of adult H3K27-altered diffuse midline glioma[J]. Basic & Clinical Medicine, 2026, 46(5): 607-613.

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成人H3K27-变异型弥漫性中线胶质瘤蛋白质组特征分析

Proteomic characteristic analysis of adult H3K27-altered diffuse midline glioma

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