PDGFRA在青少年特发性脊柱侧凸患者骨髓间充质干细胞中低表达及其对成骨分化与增殖的影响

doi:10.16352/j.issn.1001-6325.2026.06.0784

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (6): 784-790.doi: 10.16352/j.issn.1001-6325.2026.06.0784

PDGFRA在青少年特发性脊柱侧凸患者骨髓间充质干细胞中低表达及其对成骨分化与增殖的影响

张跃川¹, 汪海燕², 马帅静², 仉建国¹, 赵春华^2*, 庄乾宇^1*

1.中国医学科学院北京协和医学院北京协和医院骨科,北京 100730;
2.中国医学科学院北京协和医学院基础医学研究所人工智能细胞医药工程技术交叉创新与临床转化北京市重点实验室, 北京 100005

收稿日期:2026-02-03 修回日期:2026-03-27 出版日期:2026-06-05 发布日期:2026-05-27
通讯作者: ^*zhaochunhua@ibms.pumc.edu.cn; zhuangqianyu_pumch@126.com
基金资助:
协和人才培育计划B类项目 (UGG10250);国家自然科学基金 (82372367);北京市自然科学基金 (7232111)

Low expression of PDGFRA in bone marrow mesenchymal stem cells from patients with adolescent idiopathic scoliosis and its effects on osteogenic differentiation and proliferation

ZHANG Yuechuan¹, WANG Haiyan², MA Shuaijing², ZHANG Jianguo¹, ZHAO Chunhua^2*, ZHUANG Qianyu^1*

1. Department of Orthopedics, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730;
2. Beijing Key Laboratory of Artificial Intelligence and Cell-based Medical Engineering for Interdisciplinary Innovation and Clinical Translation, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China

Received:2026-02-03 Revised:2026-03-27 Online:2026-06-05 Published:2026-05-27
Contact: ^*zhaochunhua@ibms.pumc.edu.cn; zhuangqianyu_pumch@126.com

摘要/Abstract

摘要： 目的探讨血小板源性生长因子受体α(PDGFRA)在青少年特发性脊柱侧凸(AIS)患者骨髓间充质干细胞(BM-MSCs)中的表达特征及其对BM-MSCs成骨分化和增殖的影响。方法对既往研究BM-MSCs转录组测序数据进行分析(AIS n=12,对照 n=5),并筛选差异基因,以qRT-PCR进行验证(AIS n=12,对照 n=9)。在正常BM-MSCs中采用siRNA敲低PDGFRA。成骨诱导后通过ALP染色/活性检测、茜素红染色以及qRT-PCR和Western blot检测成骨相关指标;采用CCK-8评估增殖。结果与健康对照相比,PDGFRA在AIS患者BM-MSCs中显著低表达。PDGFRA在成骨诱导早期随分化进程上调,健康人成骨诱导6 d上调。敲低PDGFRA可同时抑制BM-MSCs早期成骨活性及晚期矿化能力,同时下调RUNX2、ALP、OPN、COL1A1、OSX、IBSP等成骨相关标志基因表达;此外,敲低PDGFRA后BM-MSCs增殖能力下降。结论 PDGFRA参与健康BM-MSCs增殖及成骨分化过程,在AIS患者BM-MSCs中低表达,可能参与AIS相关成骨不足/低骨量形成。

关键词: 青少年特发性脊柱侧凸, 骨髓间充质干细胞, PDGFRA, 成骨分化

Abstract: Objective To investigate the expression pattern of platelet-derived growth factor receptor alpha (PDGFRA) in bone marrow-derived mesenchymal stem/stromal cells (BM-MSCs) from patients with adolescent idiopathic scoliosis (AIS), and to evaluate its effects on osteogenic differentiation and proliferation of BM-MSCs. Methods Previously generated BM-MSC transcriptomic sequencing data from an established cohort were analyzed to identify differentially expressed genes (AIS, n=12; controls, n=5), and the findings were validated by qRT-PCR(AIS, n=12; controls, n=9). PDGFRA was knocked down by siRNA in normal BM-MSCs. After osteogenic induction, osteogenesis-related parameters were assessed by ALP staining/activity assay, Alizarin Red S staining, qRT-PCR, and Western blot. Cell proliferation was evaluated using CCK-8 assays. Results Compared with healthy controls, PDGFRA was significantly downregulated in BM-MSCs from patients with AIS. During the early stage of osteogenic induction, PDGFRA expression increased significantly on day 6 in healthy BM-MSCs. PDGFRA knockdown suppressed both early osteogenic activity and late mineralization in BM-MSCs, and reduced the expression of osteogenesis-related markers, including RUNX2, ALP, OPN, COL1A1, OSX, and IBSP. In addition, PDGFRA knockdown impaired the proliferation capacity of BM-MSCs. Conclusions PDGFRA is involved in the proliferation and osteogenic differentiation of BM-MSCs from healthy participants. Reduced PDGFRA expression in AIS BM-MSCs may contribute to impaired osteogenesis and low bone mass in AIS.

Key words: adolescent idiopathic scoliosis, bone marrow-derived mesenchymal stem cells, PDGFRA, osteogenic differentiation

中图分类号:

张跃川, 汪海燕, 马帅静, 仉建国, 赵春华, 庄乾宇. PDGFRA在青少年特发性脊柱侧凸患者骨髓间充质干细胞中低表达及其对成骨分化与增殖的影响[J]. 基础医学与临床, 2026, 46(6): 784-790.

ZHANG Yuechuan. Low expression of PDGFRA in bone marrow mesenchymal stem cells from patients with adolescent idiopathic scoliosis and its effects on osteogenic differentiation and proliferation[J]. Basic & Clinical Medicine, 2026, 46(6): 784-790.

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PDGFRA在青少年特发性脊柱侧凸患者骨髓间充质干细胞中低表达及其对成骨分化与增殖的影响

Low expression of PDGFRA in bone marrow mesenchymal stem cells from patients with adolescent idiopathic scoliosis and its effects on osteogenic differentiation and proliferation

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