关键词: mir-150, 骨髓间充质干细胞, CXCR4

Abstract: Objective To study whether miR-150 can regμLate the expression of CXCR4 in mesenchymal stem cells of diabetic nephropathy. Method Flow cytometry was used to evaluate the CD phenotype on the surface of MSCs. Adipogenic, osteogenic differentiation of MSCs were detected by fluorescence microscopy, real-time RT -PCR was used to detect the expression of miR-150 in MSCs. Also, the expressions of CXCR4, and SDF-1 were tested by Western blot. Transwell migration experiment was perform to detected MSCs migration Results : MSCs expressed positively the CD29（97.6%±2.2%）, CD90 （99.1%±0.6%）,and negatively the CD45（17.3%±4.5%）. MSCs had the potency of differentiating into fat, bone cells. The expression of miR-150 in MSCs was reduced in DN compared with normal group, However, the expressions of CXCR4 and SDF-1 were declined in miR-150 over-expressed MSCs. Moreover，the numbers of MSCs migration were increased in miR-150 inhibitor compared with control group. Conclusions：The change of miR-150 in MSCs from DN may be an important factor to migration related protein expression.

Key words: mir-150, MSC, CXCR4