Abstract: Objective To investigate the effects of Chliroquine on Pentylenetetrazole(PTZ)- activitied hippocampal astrocyte of rats. Methods Neonatal rat (24h) hippocampal astrocytes were obtained and divided into contron group, PTZ-activitied group and Chliroquine groups with Chliroquine treatment at 25, 50, 75mg/ L. The cell proliferation, expression of glial fibrillary acidic protein (GFAP) and CyclinD1 after the treatments were detected with MTT assay, immunofluorescence cytochemistry and western bloting respectively. Results In comparison with PTZ-activition group, Chliroquine treatment resulted in significant inhibition of the cell proliferation, GFAP and CyclinD1 in the PTZ-activated astrocytes (P<0.05). Chliroquine at 75mg/L showed the strongest inhibitory effects against astrocyte activation and maintained nearly normol level of astrocyte activation in comparison with the control group. Conclusion Chliroquine could inhibited the activation of astrocytes which activitied by PTZ. So Chliroquine may be used to treat epilepsy.