Dynamic changes of urinary proteomics and metabolomics in chronic hypoxia mouse model

doi:10.16352/j.issn.1001-6325.2026.04.0484

Abstract

Abstract: Objective To detect changes in the metabolome and proteome of urine in a mouse model of chronic hypoxia and to explore the correlation between the urinary proteome and metabolome. Methods Six C57BL/6J male mice were placed in an oxygen chamber with 14% oxygen concentration for 5 weeks of hypoxic exposure. Mouse urine was collected weekly with a control group established simultaneously. Non-targeted proteomics and metabolomics techniques were employed for dynamic analysis of urine samples. Results Compared to the control group, the urine of chronic hypoxia mice contained 458 differentially expressed proteins and 718 differential metabolites. These proteins and differential metabolites were functionally enriched primarily in glycolysis pathway, lysosomal pathway, and cholesterol synthesis pathway. Joint analysis of proteomics and metabolomics revealed that proteins with high correlation to differential metabolites were mainly enriched in PI3K-AKT, complement and coagulation cascades, and cell cycle pathways. Conclusions Urinary proteomics and metabolomics may reflect the changes induced by chronic hypoxic stimulation.

Key words: chronic hypoxia, urine, proteomics, metabolomics

CLC Number:

R363

LIU Tingting, HE Mengzhen, ZHANG Yuxue, HU Siqi, LI Jun, SUN Wei. Dynamic changes of urinary proteomics and metabolomics in chronic hypoxia mouse model[J]. Basic & Clinical Medicine, 2026, 46(4): 484-490.

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