Abstract: Objective To investigate the relationship between polymorphism of the APOB gene and stroke in Han Chinese population．Methods This study was a population-based cross-sectional case-control study. In APOB gene, the polymorphisms of C7673T and 5′I/D were determined by Polymerase Chain Restriction-Restriction Fragment Length Polymorphism (PCR-RFLP) and sequencing in 547 patients with ischemie stroke (466 ischemie stroke) and 886 controls． Results The distributions of C7673T (p=0.6293) and 5′I/D (p=0.5359) genotype frequencies in men and women were in accordance with the Hardy-Weinberg equilibrium. In C7673T locus, the genotype CC frequency in females was significant lower in ischemic stroke than that in healthy controls (p＜0.01), and allele C frequency was significant lower in ischemic stroke than that in healthy controls (p＜0.01). But significant differences were not found in males. The frequency of genotype II at 5′I/D locus in males was significant lower in cerebral hemorrhage patients than that in healthy controls (p＜0.01). The frequency of genotype II and the allele I at 5′I/D locus in males was significant lower in ischemic stroke patients than that in healthy controls（p＜0.01）. The frequency of genotype II and the allele I at 5′I/D locus in females was also significant lower in ischemic stroke patients than that in healthy controls, （p＜0.01，p＜0.05，respectively）Conclusion The C7673T polymorphism of APOB gene associates with ischemic stroke in Fangshan district in female. The 5′I/D polymorphism of APOB gene associates with ischemic stroke in Fangshan district in male and female.

Key words: ApoB gene, stroke, cerebral hemorrhage, ischemic stroke, polymorphism