Abstract: Objective 　To investigate the polymorphism of seventeen Y chromosomal short tandem repeat (STR) loci in dong, miao and zhuang nationality of Guangxi of China，and then evaluate their forensic application．Method the DNA was extracted from oral swab of minority population with magnetic beads method and then seventeen Y-STR loci were amplified with AmpFlSTR? Yfiler? PCR Amplification Kit System . The PCR products were detected with 3l30XL Genetic Analyzer. The allele frequencies，diversities and genetic distance were calculated ．Results The information of allele distribution and gene diversity of the 17 Y-STR loci were obtained from three minority population.275 haplotypes were found in three minority population. The haplotype diversity of dong nationality is 0.9972,Miao is 0.9966,zhuang is 0.9964.The genetic distance between dong and miao nationality is most close. Conclusion The haplotype diversity of 17 Y-STR loci is so effective in racial identification, paternity identification and human population genetics.

Key words: Y chromosome, haplotype, genetic polymorphism, genetic distanc, STR