LINC00339通过调控miR-15b-5p/E2F3轴促进人甲状腺癌细胞系的迁移和侵袭

doi:10.16352/j.issn.1001-6325.2026.09.1175

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (9): 1175-1182.doi: 10.16352/j.issn.1001-6325.2026.09.1175

LINC00339通过调控miR-15b-5p/E2F3轴促进人甲状腺癌细胞系的迁移和侵袭

张友梁, 任光玉, 吴嘉, 朱皓皞^*

中国人民解放军联勤保障部队第九〇八医院病理科,江西南昌 330002

收稿日期:2025-07-23 修回日期:2025-11-03 出版日期:2026-09-05 发布日期:2026-08-18
通讯作者: ^*2572839187@qq.com
基金资助:
联勤保障部队第908医院苗子基金(YNKT202414,YNKT2025002)

LINC00339 promotes the migration and invasion of thyroid cancer cell lines by regulating the miR-15b-5p/E2F3 axis

ZHANG Youliang, REN Guangyu, WU Jia, ZHU Haohao^*

Department of Pathology, the 908th Hospital of the Joint Logistic Support Force of the People′s Liberation Army of China, Nanchang 330002, China

Received:2025-07-23 Revised:2025-11-03 Online:2026-09-05 Published:2026-08-18
Contact: ^*2572839187@qq.com

摘要/Abstract

摘要： 目的探讨长链非编码RNA LINC00339在甲状腺癌(TC)细胞迁移和侵袭中的作用及其分子机制。方法将甲状腺癌细胞系KTC-1进行分组:sh-NC组、sh-LINC00339组、NC mimic组、miR-15b-5p mimic组、NC inhibitor组、miR-15b-5p inhibitor组、sh-LINC00339+NC inhibitor组、sh-LINC00339+miR-15b-5p inhibitor组、miR-15b-5p mimic+pcDNA-NC组、miR-15b-5p mimic+pcDNA-E2F3组、sh-LINC00339+pcDNA-NC组和sh-LINC00339+pcDNA-E2F3组。每组设置3个复孔。通过RT-qPCR检测LINC00339、miR-15b-5p和E2F转录因子3(E2F3) mRNA的表达水平;利用划痕实验和Transwell小室法评估细胞迁移和侵袭能力;瘤球形成实验检测干细胞特性;蛋白质免疫印迹检测细胞干性相关标志物的蛋白质表达。结果与正常甲状腺细胞相比,TC细胞系中LINC00339和E2F3表达上调,miR-15b-5p表达下调(P＜0.05)。干扰LINC00339可降低E2F3表达,抑制细胞迁移、侵袭和干细胞特性,同时上调miR-15b-5p。抑制miR-15b-5p可部分逆转这些效应(P＜0.05)。过表达miR-15b-5p可抑制E2F3表达和细胞迁移、侵袭及干细胞特性,而过表达E2F3则可恢复这些效应(P＜0.05)。结论 LINC00339在TC细胞系中呈高表达,可通过靶向下调miR-15b-5p表达,解除miR-15b-5p对E2F3的抑制作用,从而诱导TC细胞系的干细胞特性,进而促进细胞迁移和侵袭。

关键词: LINC00339, miR-15b-5p, E2F转录因子3, 甲状腺癌, 细胞干性

Abstract: Objective To investigate the role of long non-coding RNA LINC00339 in the migration and invasion of thyroid cancer (TC) cells and its molecular mechanism. Methods Thyroid cancer cell line KTC-1 was divided into sh-NC group, sh-LINC00339 group, NC mimic group, miR-15b-5p mimic group, NC inhibitor group, miR-15b-5p inhibitor group, sh-LINC00339+NC inhibitor group, sh-LINC00339+miR-15b-5p inhibitor group, miR-15b-5p mimic+pcDNA-NC group, miR-15b-5p mimic+pcDNA-E2F3 group, sh-LINC00339+pcDNA-NC group and sh-LINC00339+ pcDNA-E2F3 group. Three holes were set in each group. The expression levels of LINC00339, miR-15b-5p and E2F3 mRNA were detected by RT-qPCR. Wound healing assay and Transwell assay were used to evaluate the ability of cell migration and invasion. Tumor sphere formation assay was used to detect the characteristics of stem cells. Western blot was used to detect the protein expression of stemness-related markers. Results Compared with normal thyroid cells, the expression of LINC00339 and E2F3 in TC cell lines was up-regulated, and the expression of miR-15b-5p was down-regulated (P＜0.05). Interfering with LINC00339 can reduce the expression of E2F3, inhibit cell migration, invasion and stem cell characteristics, and up-regulate miR-15b-5p. Inhibition of miR-15b-5p partially reversed these effects (P＜0.05). Over-expression of miR-15b-5p could inhibit E2F3 expression and cell migration, invasion and stem cell characteristics, while over-expression of E2F3 could restore these effects (P＜0.05). Conclusions LINC00339 is highly expressed in TC cell lines, which can relieve the inhibitory effect of miR-15b-5p on E2F3 by targeting down-regulation of miR-15b-5p expression, thereby inducing the stem cell characteristics of TC cell lines and promoting cell migration and invasion.

Key words: LINC00339, miR-15b-5p, E2F transcription factor 3, thyroid carcinoma, cell stemness

中图分类号:

R736.1

张友梁, 任光玉, 吴嘉, 朱皓皞. LINC00339通过调控miR-15b-5p/E2F3轴促进人甲状腺癌细胞系的迁移和侵袭[J]. 基础医学与临床, 2026, 46(9): 1175-1182.

ZHANG Youliang, REN Guangyu, WU Jia, ZHU Haohao. LINC00339 promotes the migration and invasion of thyroid cancer cell lines by regulating the miR-15b-5p/E2F3 axis[J]. Basic & Clinical Medicine, 2026, 46(9): 1175-1182.

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LINC00339通过调控miR-15b-5p/E2F3轴促进人甲状腺癌细胞系的迁移和侵袭

LINC00339 promotes the migration and invasion of thyroid cancer cell lines by regulating the miR-15b-5p/E2F3 axis

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