关键词: miR-130a-3p, 宫颈癌细胞, 顺铂耐药, 放化疗

Abstract: Objective To explore the impact of miR-130a-3p on the sensitivity of human cervical cancer cell line Caski to chemoradiotherapy. Methods The cells were divided into four groups: the control group (Caski cell line), cisplatin-resistant group (Caski/DDP cells), miR-130a-3p overexpression group (Caski+miR-130a-3p cells), and cisplatin-resistant miR-130a-3p overexpression group (Caski/DDP+miR-130a-3p cells). After treatment with various concentrations of cisplatin and radiotherapy, cell proliferation was assessed using the CCK-8 assay, cell apoptosis was measured by flow cytometry, and the expression of ZEB1 protein was detected by Western blot. Results After overexpression of miR-130a-3p, the IC₅₀ of cisplatin in Caski cells decreased significantly (9.447 μg/mL vs. 6.429 μg/mL, P＜0.05), and the apoptosis rate increased, although the difference was not statistically significant (mean apoptosis rate 29.3% vs. 30.6%, P＞0.05), and the expression of ZEB1 was significantly downregulated (P＜0.01). In Caski/DDP cell strain, the IC₅₀ decreased more markedly after overexpression of miR-130a-3p (IC₅₀: 58.38 μg/mL vs. 18.38 μg/mL, P＜0.000 1); the rate of cell apoptosis increased significantly (mean apoptosis rate: 15.9% vs. 29.8%, P＜0.001), and the expression of ZEB1 protein was downregulated (P＜0.01). Conclusions Overexpression of miR-130a-3p may increase the chemoradiotherapy sensitivity of cervical cancer cells by downregulating ZEB1 expression.

Key words: miR-130a-3p, cervical cancer cell, ciplatin resistance, radiochemotherapy