非哺乳期乳腺炎患者外周血及单核细胞来源HMGB1水平与病情相关

doi:10.16352/j.issn.1001-6325.2026.06.0855

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (6): 855-860.doi: 10.16352/j.issn.1001-6325.2026.06.0855

非哺乳期乳腺炎患者外周血及单核细胞来源HMGB1水平与病情相关

耿睿婕¹, 姑丽尼格尔·库来西², 张建军², 贾尔肯阿依·阿不都拉依², 高扬², 寇新红^1*

1.乌鲁木齐市友爱医院乳腺甲状腺科, 新疆乌鲁木齐 831499;
2.乌鲁木齐市妇幼保健院乳腺科, 新疆乌鲁木齐 830001

收稿日期:2025-06-03 修回日期:2025-09-24 出版日期:2026-06-05 发布日期:2026-05-27
通讯作者: ^*1804926885@qq.com
基金资助:
乌鲁木齐市卫生健康委科技计划项目(202217);新疆维吾尔自治区卫生健康青年医学科技人才专项(WJWY-202313)

Levels of HMGB1 in peripheral blood and monocyte-derived HMGB1 correlate with disease condition in patients with non-puerperal mastitis

GENG Ruijie¹, Gulinigeer KULAIXI², ZHANG Jianjun², Jiaerkenayi ABUDULAYI², GAO Yang², KOU Xinhong^1*

1. Department of Breast and Thyroid, Urumqi Youai Hospital, Urumqi 831499;
2. Department of Breast, Urumqi Maternal and Child Health Hospital, Urumqi 830001, China

Received:2025-06-03 Revised:2025-09-24 Online:2026-06-05 Published:2026-05-27
Contact: ^*1804926885@qq.com

摘要/Abstract

摘要： 目的探究非哺乳期乳腺炎(NPM)患者外周血及单核细胞来源高迁移率族蛋白B1(HMGB1)水平变化及与病情的相关性。方法共83例NPM患者根据病情分为急性组(acute)42例和慢性组(chronic)41例,另以40例健康受试者作为对照组(control)。采用ELISA检测各组的外周血HMGB1、C反应蛋白(CRP)、白细胞介素6(IL-6)水平;流式细胞测量术检测单核细胞的数量及HMGB1水平;qPCR和Western blot检测单核细胞中HMGB1表达;Pearson法分析单核细胞的HMGB1表达与外周血CRP、IL-6水平的相关性。绘制ROC曲线评估单核细胞的HMGB1表达水平对NPM病情的诊断价值。结果 NPM患者的外周血HMGB1、CRP、IL-6水平、M1型单核细胞数量及单核细胞的HMGB1 mRNA均高于对照组,同时急性组高于慢性组(P＜0.05)。单核细胞的HMGB1 mRNA水平与外周血CRP(r=0.426)、IL-6(r=0.569)呈正相关(P均＜0.05)。M1型单核细胞的HMGB1蛋白水平诊断NPM的AUC为0.739(95% CI:0.67～0.82),敏感度与特异度分别为69.8%和77.2%;评估病情的AUC为0.828(95% CI:0.73～0.91),敏感度与特异度分别为83.3%和71.4%。结论 NPM患者外周血单核细胞上HMGB1表达水平增加,且其与外周血CRP、IL-6水平呈正相关,有助于NPM诊断及病情评估。

关键词: 非哺乳期乳腺炎, 单核细胞, 高迁移率族蛋白B1, 诊断

Abstract: Objective To investigate the changes in peripheral blood and monocyte-derived high mobility group protein B1 (HMGB1) levels in patients with non-puerperal mastitis (NPM) and their correlation with the disease. Methods A total of 83 patients with NPM were divided into the acute group (42 cases) and the chronic group (41 cases) according to their conditions Additionally, 40 healthy subjects were taken as the control group. The levels of HMGB1, C-reactive protein (CRP), and interleukin-6 (IL-6) in the peripheral blood of each group were detected by ELISA. The number of monocytes and the level of HMGB1 were detected by flow cytometry; the expression of HMGB1 in monocytes was detected by qPCR and Western blot; the correlation between the expression of HMGB1 in monocytes and the levels of CRP and IL-6 in peripheral blood was analyzed by the Pearson method. ROC curves were constructed to evaluate the diagnostic value of HMGB1 expression level in monocytes for the condition of NPM. Results The levels of HMGB1, CRP, IL-6 in the peripheral blood of patients with NPM, the number of M1-type monocytes and the HMGB1 mRNA expression in monocytes were all higher than those in the control group. Meanwhile, these levels in the acute group were higher than those in the chronic group (P＜0.05). The level of HMGB1 mRNA in monocytes was positively correlated with CRP (r=0.426) and IL-6 (r=0.569) in peripheral blood (all P＜0.05). The AUC of HMGB1 protein level in M1 monocytes for diagnosing NPM was 0.739 (95% CI: 0.67-0.82), the sensitivity and specificity were 69.8% and 77.2%. The AUC for evaluating the disease condition was 0.828 (95% CI: 0.73-0.91), the sensitivity and specificity were 83.3% and 71.4%. Conclusions The expression of HMGB1 in monocytes of patients with NPM increases and is positively correlated with the levels of peripheral blood CRP and IL-6, which is helpful for the diagnosis and condition assessment of NPM.

Key words: non-puerperal mastitis, monocyte, high mobility group protein B1, diagnosis

中图分类号:

R655.8

耿睿婕, 姑丽尼格尔·库来西, 张建军, 贾尔肯阿依·阿不都拉依, 高扬, 寇新红. 非哺乳期乳腺炎患者外周血及单核细胞来源HMGB1水平与病情相关[J]. 基础医学与临床, 2026, 46(6): 855-860.

GENG Ruijie. Levels of HMGB1 in peripheral blood and monocyte-derived HMGB1 correlate with disease condition in patients with non-puerperal mastitis[J]. Basic & Clinical Medicine, 2026, 46(6): 855-860.

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非哺乳期乳腺炎患者外周血及单核细胞来源HMGB1水平与病情相关

Levels of HMGB1 in peripheral blood and monocyte-derived HMGB1 correlate with disease condition in patients with non-puerperal mastitis

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