关键词: 骨髓间充质干细胞, 慢病毒, 细胞增殖, 细胞分化

Abstract: Objective To explore the effects of lentivirus-mediated GDNF on biologic characteristics of cynomolgus monkey mesenchymal stem cells. Methods Under sterile condition, bone marrow mononuclear cells of cynomolgus monkey were isolated by density-gradient centrifugation and MSCs were cultured in vitro. GDNF lentivirus infected cynomolgus monkey MSCs (cMSCs). Using ELISA, Real-time PCR, flow cytometry (FCM), BrdU incorporation and other experimental methods, GDNF gene expression, cell surface markers, proliferation and differentiation of cynomolgus monkey MSCs were observed before and after the infection of lentivirus. Results cMSCs highly expressed GDNF in vitro after infection of lentivirus. After the infection of GDNF lentivirus, cell morphology and adipocyte differentiation of cMSCs did not change significantly, but cell surface markers CD34, CD90 and Stro-1 positive cells increased in vitro, and the proliferative potential decreased. Conclusion The cell surface markers and the proliferative potential of cMSCs changed after the infection of GDNF lentivirus, but the adipocyte differentiation ability of MSCs had no effect.

Key words: Bone mesenchymal stem cells, lentivirus, cell proliferation, cell differentiation