马里苷减轻高糖诱导的大鼠心肌细胞系H9c2损伤

doi:10.16352/j.issn.1001-6325.2024.09.1269

基础医学与临床 ›› 2024, Vol. 44 ›› Issue (9): 1269-1273.doi: 10.16352/j.issn.1001-6325.2024.09.1269

马里苷减轻高糖诱导的大鼠心肌细胞系H9c2损伤

祖力皮亚·阿布拉¹, 赵强², 张泰民³, 李甜^4,5*

新疆医科大学 1.中医学院;3.临床医学部;4.基础医学院组织学胚胎学教研室;5.新疆地方病分子生物学重点实验室,新疆乌鲁木齐 830001;
2.新疆维吾尔自治区人民医院心脏及泛血管医学诊疗中心,新疆乌鲁木齐 830001

收稿日期:2024-01-03 修回日期:2024-05-15 出版日期:2024-09-05 发布日期:2024-08-30
通讯作者: ^*292629092@qq.com
基金资助:
新疆维吾尔自治区高校科研计划(XJEDU2020Y021);国家级大学生创新创业训练计划(202010760011);新疆医科大学博士启动基金(2023)

Marein alleviates high glucose-induced damage of rat myocardial cell line H9c2

Zulipiya·ABULA¹, ZHAO Qiang², ZHANG Taimin³, LI Tian^4,5*

1. School of Traditional Chinese Medical; 3. Clinical Medicine Department; 4. Department of Histology and Embryology, School of Basic Medicine; 5. Xinjiang Key Laboratory of Molecular Biology of Endemic Diseases, Xinjiang Medical University, Urumqi 830001;
2. Heart and Pan-vascular Medical Diagnosis and Treatment Center, People′s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China

Received:2024-01-03 Revised:2024-05-15 Online:2024-09-05 Published:2024-08-30
Contact: ^*292629092@qq.com

摘要/Abstract

摘要： 目的研究马里苷对高糖所致大鼠心肌细胞系(H9c2)损伤的影响。方法将H9c2细胞分为对照组(Ctrl)、高糖(30 mmol/L)损伤模型组和马里苷(25、50、100 μmol/L)干预模型组。MTT法检测H9c2细胞的活性;Western blot检测细胞LC-3Ⅱ/Ⅰ、p62、mTOR蛋白表达。结果与对照组相比,模型组细胞活性下降(P＜0.01),马里苷组H9c2细胞活性显著增强(P＜0.01);模型组LC-3Ⅱ/Ⅰ蛋白表达水平降低(P＜0.05),p62及mTOR水平升高(P＜0.01),马里苷组LC-3Ⅱ/Ⅰ水平增加,p62及mTOR降低(P＜0.01),且随着浓度的增加蛋白质表达量更加明显。结论马里苷能够影响高糖诱导的心肌细胞损伤的自噬相关蛋白质的表达。

关键词: 马里苷, H9c2细胞, 高糖损伤, 自噬

Abstract: Objective To study the effect of marein on the damage of rat myocardial cell line (H9c2) induced by high glucose. Methods H9c2 cells were divided into Ctrl group, high glucose (30 mmol/L) injury model group and marein (25, 50, 100 μmol/L) intervention group. The viability of H9c2 cells was detected by MTT method. Western blot was used to detect the protein expression level of LC-3Ⅱ/Ⅰ,p62 and mTOR. Results Compared with the Ctrl group, the viability of H9c2 cells in the model group was decreased (P＜ 0.01) and the viability of H9c2 cells in the marein group was significantly increased (P＜ 0.01). The protein expression of LC-3Ⅱ/Ⅰ in model group was decreased (P＜ 0.05) and the level of p62 and mTOR was increased (P＜ 0.01). The level of LC-3Ⅱ/Ⅰ in the marein group was increased, while the level of p62 and mTOR was decreased (P＜ 0.01). The protein expression was more obvious with the increase of concentration. Conclusions Marein can affect the expression of autophagy associated proteins in cardiomyocyte injury induced by high glucose.

Key words: marein, H9c2 cell, high-glucose injury, autophagy

中图分类号:

R285.5

祖力皮亚·阿布拉, 赵强, 张泰民, 李甜. 马里苷减轻高糖诱导的大鼠心肌细胞系H9c2损伤[J]. 基础医学与临床, 2024, 44(9): 1269-1273.

Zulipiya·ABULA, ZHAO Qiang, ZHANG Taimin, LI Tian. Marein alleviates high glucose-induced damage of rat myocardial cell line H9c2[J]. Basic & Clinical Medicine, 2024, 44(9): 1269-1273.

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马里苷减轻高糖诱导的大鼠心肌细胞系H9c2损伤

Marein alleviates high glucose-induced damage of rat myocardial cell line H9c2

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