miR-451通过Akt负向调控糖尿病肾病患者组织上皮间质转分化

基础医学与临床 ›› 2020, Vol. 40 ›› Issue (4): 469-472.

miR-451通过Akt负向调控糖尿病肾病患者组织上皮间质转分化

郭红宝, 刘友霞, 贾俊亚, 闫铁昆^*

天津医科大学总医院肾脏内科,天津 300052

收稿日期:2019-03-08 修回日期:2019-12-06 出版日期:2020-04-05 发布日期:2020-04-06
通讯作者: *703571348@qq.com
基金资助:
国家自然基金青年基金(8160030684)

miR-451 negatively regulates EMT of the diabetic nephropathy by Akt

GUO Hong-bao, LIU You-xia, JIA Jun-ya, YAN Tie-kun^*

Department of Nephrogy, Tianjin Medical University General Hospital, Tianjin 300052, China

Received:2019-03-08 Revised:2019-12-06 Online:2020-04-05 Published:2020-04-06
Contact: *703571348@qq.com

摘要/Abstract

摘要： 目的研究微小RNA-451(miR-451)对糖尿病肾病组织上皮间质转分化(EMT)的影响。方法取健康人肾脏组织和糖尿病患者肾脏组织,RT-qPCR检测肾组织mi-451、Akt和EMT相关基因(E-钙黏素、波形蛋白和α-平滑肌肌动蛋白)的表达;Western blot和免疫组织化学染色(IHC)检测Akt和EMT相关蛋白的表达。结果糖尿病肾病肾脏组织中miR-451和E-钙黏素mRNA表达量显著低于正常肾组织(P＜0.05);Akt、vimentin和α-SMA mRNA表达量显著增多(P＜0.05);Akt、vimentin和α-SMA蛋白表达量显著增高(P＜0.05);E-钙黏素蛋白表达量显著降低(P＜0.05)。结论糖尿病肾病时miR-451很可能通过Akt负向影响肾脏上皮间质转分化。

关键词: 糖尿病肾病, 微小RNA-451, 上皮间质转分化

Abstract: Objective To study microRNA-451 (miR-451)'s influence on diabetic nephropathy epithelial mesenchymal transdifferentiation (EMT). Methods Taking human healthy kidney tissues and diabetic nephropathy kidney tissues, real-time PCR was used to detect the expression of miR-451, Akt and EMT related genes (E-cadhering, vimentin and alpha smooth muscle actin)in different groups; Western blot and immuno-histochemical staining (IHC) were used to detect the expression of Akt and EMT related proteins in different groups. Results The expressions of miR-451 and E-cadherin mRNA significantly reduced in diabetic nephropathy kidney tissues as compared with healthy kidney tissues, and the expressions of Akt, vimentin and alpha-SMA mRNA significantly increased. The expressions of Akt, vimentin and alpha-SMA protein significantly increased, and the expression of E-cadherin protein decreased significantly (P＜0.05). Conclusions The miR-451 is likely to negatively influence diabetic nephropathy epithelial mesenchymal trans differentiation by Akt.

Key words: diabetic nephropathy, miR-451, epithelial-mesenchymal transformation

中图分类号:

郭红宝, 刘友霞, 贾俊亚, 闫铁昆. miR-451通过Akt负向调控糖尿病肾病患者组织上皮间质转分化[J]. 基础医学与临床, 2020, 40(4): 469-472.

GUO Hong-bao, LIU You-xia, JIA Jun-ya, YAN Tie-kun. miR-451 negatively regulates EMT of the diabetic nephropathy by Akt[J]. Basic & Clinical Medicine, 2020, 40(4): 469-472.

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