关键词: 干扰素诱导蛋白, p204, 慢病毒载体, 转染, 成纤维细胞, 大鼠

Abstract: Objective To observe the effect of transfection with lentivirus carrying ifi204 genes on the expression of p204 in rat vascular adventitial fibroblasts. Methods By means of culturing the vascular adventitial fibroblasts of rat aortic,and then conducting Immunocytochemistry to identify the type and purity of these rat fibroblasts. The vascular adventitial fibroblasts of rat aortic cultured in vitro were transfected with lentivirus vector system carrying Ifi204 genes(Ifi204 group).The fibroblasts which has been transfected with the empty vector lentivirus particles as the control group (group C)，and the untreated fibroblasts as the blank group (group N). Test the expression of mRNA and protein of p204 in the fibroblasts by real-time fluorescent quantitative PCR and Western blot test separately .Results There was expression of p204 in group N. Compared with group N and group C, mRNA and protein expression of p204 in the Ifi204 group had been all up-regulated (P <0.01). Conclusions There was structural expression of p204 in vascular adventitial fibroblasts of rat aortic. Transfection with lentivirus carrying Ifi204 genes can up-regulate the expression of p204 in vascular adventitial fibroblasts.

Key words: interferon-induced protein, p204, lentivirus vector, transfection, fibroblast, rats