关键词: miR-31, LATS2, 心肌细胞, 肥大

Abstract: ObjectiveTo observe the expression of miR-31 and its target gene LATS2 in the hypertrophy process of rat cardiomyocytes.MethodsTargeting regulation of miR-31 on LATS2 was identified by a dual luciferase gene reporter system. Rat primary cardiomyocytes were isolated and cultured in vitro, and hypertrophic model of cardiomyocytes was constructed by administering 10-6mol/L AngII. MiR-31,LATS2 and hypertrophy genes ANP,β-MHCwere detected by RT-qPCR.Morphology of the cardiomyocyteswas observed by fluorescence staining to F-actin.LATS2 protein was further detected by western blot.ResultsMiR-3l could specifically bind to 3'UTR of LATS2,which made the luciferase activity decrease significantly.Hypertrophy genes ANP and β-MHCwere up-regulated at 48 hour and the area of cardiomyocytes was increased significantly at 96 hour after administration of 10-6mol/LAngII. Accompanied with the hypertrophic changes of cardiomyocytes, miR-31 was significantly up-regulated, while LATS2 in gene and protein levels were reduced obviously.ConclusionsAccompanied with the hypertrophic changes of rat cardiomyocytes, miR-31 was significantly up-regulated, while LATS2 in gene and protein levels were reduced obviously.

Key words: miR - 31, LATS2, cardiomyocytes, hypertrophy