关键词: [关键字]：食管肿瘤, Lats1, YAP, 增殖 , 凋亡

Abstract: Objective To discuss the impact and mechanism on proliferation,apoptosis and cell cycle in Eca109 cells by overexpress LATS1 in lentivirus transfection technique. Methods Built LATS1-lentivirus vector then transfected the lentivirus vector into Eca109 cells；LATS1 mRNA expression was detected by real-time PCR；LATS1、YAP、BAX/BCL-2 protein expression were detected by western blotting；proliferation ability was detected by MTS assay；the ratio of apoptosis and cell cycle were detected by FCM；the dyeing of apoptosis was observed by hochest33258. Result After transfection of LATS1-lentivirus in Eca109 cells（Ad-LATS1 group），we found lats1 mRNA expression and protein expression level，BAX protein expression level were remarkablely higher than control group and Ad-GFP groups while YAP,BCL-2 protein expression level were on the contrary（P＜0.05）；The ratio of proliferation in Ad-LATS1 group was notable lower than control group and Ad-GFP groups from the fifth day（P＜0.05）；FCM showed more cells stay in G1 phase in Ad-LATS1 group than in control group and-GFP groups while the ratio of cells in S phase were on the contrary and apoptosis rate was much higher in Ad-LATS1 group than in others（P＜0.05）；Hoechst 33258 dying also demonstrated that LATS1 could induce apoptosis in Eca109 cells through the degree and range of dyeing. Conclusion gene LATS1could up-regulate BAX whlle down-regulate YAP and BCL-2 to increase the apoptosis rate of Eca109 cells，more over，it could prolong the G1 phase of cell cycle and shorten the S phase to inhibit the proliferation of Eca109 cells.

Key words: [Key words]：esophagus tumor, Lats1, YAP, proliferation , apoptosis