关键词: K562细胞, 慢病毒, pirin, 红系分化, 白血病

Abstract: Objective This article is aimed at researching the influence of pirin knocking down on the differentiation and proliferation of K562 cells. Methods Techniques of shRNA interference was applied to generate stable cell strain of pirin low expression. Western blot was used to analyse the interference efficiency of pirin-shRNA. MTT and benzidine staining were applied to detect the cell viability and hemoglobin synthesis of the stable cell line in the presence of inducers Results Western blot showed that the pirin knockdown stable cell strain had a lower expression of pirin compared to the control group, MTT results showed that there was no difference between the pirin knockdown cell strain and the control group, but the hemoglobin synthesis had been reduced as compared to the control group. Conclusions pirin knocking down will not affect the cell viability of K562 cells, but it can inhibit the erythroid differentiation of K562 cells.

Key words: K562 cells, Lentivirus, pirin, erythroid differentiation, leukemia