慢性应激致大鼠抑郁行为涉及海马形态变化和BDNF表达降低

基础医学与临床 ›› 2011, Vol. 31 ›› Issue (5): 565-569.

慢性应激致大鼠抑郁行为涉及海马形态变化和BDNF表达降低

王涵¹,李娜¹,文威¹,姜蓉¹,周岐新¹,²

1. 重庆医科大学
2.

收稿日期:2010-09-29 修回日期:2011-02-25 出版日期:2011-05-05 发布日期:2011-05-06
通讯作者: 周岐新 E-mail:cqzhouqx@yahoo.com.cn

Depressive behavior of rats induced by CUMS involved in abnormal pathomorphology and BDNF expression in hippocampus of rats

Han WANG¹,Na LI²,Wei WEN²,Rong JIANG²,Qi-xin ZHOU¹,²

1. Chongqing Medical University
2.

Received:2010-09-29 Revised:2011-02-25 Online:2011-05-05 Published:2011-05-06
Contact: Qi-xin ZHOU E-mail:cqzhouqx@yahoo.com.cn

摘要/Abstract

摘要： 目的研究慢性不可预见轻度刺激（CUMS）致大鼠抑郁模型与海马病理形态改变和脑源性神经营养因子（BDNF）表达异常的关系。方法大鼠分为对照组和模型组，应用CUMS建立大鼠抑郁模型；以开场（open-field）法、黄嘌呤氧化法和硫代巴比妥酸法、HE染色和免疫组化法分别检测大鼠行为、皮质MDA水平和SOD活力、海马病理形态和海马BDNF表达。结果与对照大鼠相比，接受28d CUMS大鼠开场实验得分明显降低，皮层SOD活力显著下降和MDA含量明显增高，海马神经元呈明显核固缩和BDNF表达显著降低(吸光度值803±422 vs 237±96，p<0.01)。结论 CUMS致大鼠明显抑郁行为，其机制可能与神经元氧化-抗氧化系统失衡，自由基生成增加，由此致海马神经元受损和BDNF表达减少有关。

关键词: 抑郁症, 慢性不可预见轻度刺激, BDNF, 海马

Abstract: Objective Establish depression model of rat through chronically unpredicted mild stress（CUMS）in order to investigate its connection with the changes of hippocampal pathomorphology and Brain-derived neurotrophic factor（BDNF）. Methods Rats were randomly divided into two groups,control and model.CUMS was used to establish depression model of rats. The rat behavior, SOD activity and MDA level of cortex, hippocampal pathomorphology, and BDNF expression were tested by the methods of open-field, xanthine oxidase, TBA,HE staining,and immunohistochemisty, respectively. Results It was shown that there existed significantly decreasing open-field scores，SOD activity，and BDNF expression(A value 803±422 vs 237±96，p<0.01) with obviously increasing MDA level and karyopyknosis of hippocampal neurocytes in rats treated by CUMS，compared with control rats. Conclusions Depression behavior of rats can be induced by CUMS，the mechanism of which may be associated with damage of hippocampal neurocytes and decrease of BDNF expression from imbalance of oxidation-antioxidation system and increase of free radicals in rat brains.

Key words: BDNF

中图分类号:

王涵李娜文威姜蓉周岐新. 慢性应激致大鼠抑郁行为涉及海马形态变化和BDNF表达降低 [J]. 基础医学与临床, 2011, 31(5): 565-569.

Han WANG Na LI Wei WEN Rong JIANG Qi-xin ZHOU. Depressive behavior of rats induced by CUMS involved in abnormal pathomorphology and BDNF expression in hippocampus of rats[J]. Basic & Clinical Medicine, 2011, 31(5): 565-569.

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