Apelin-13对离体缺血/再灌注大鼠心脏损伤的影响

基础医学与临床 ›› 2007, Vol. 27 ›› Issue (8): 864-867.

Apelin-13对离体缺血/再灌注大鼠心脏损伤的影响

曾翔俊王红霞芦玲巧王晓燕马利权张立克唐朝枢

首都医科大学首都医科大学首都医科大学首都医科大学首都医科大学首都医科大学北京大学第一医院心血管病研究所

收稿日期:2006-09-08 修回日期:2006-10-13 出版日期:2007-08-25 发布日期:2007-08-25
通讯作者: 曾翔俊

Protective effects of apelin-13 on the ischemia/reperfusion injury in rat heart

Xiang-jun Zeng Hong-xia Wang Ling-qiao Lu Xiao-yan Wang Li-quan Ma Li-ke Zhang Chao-shu Tang

Received:2006-09-08 Revised:2006-10-13 Online:2007-08-25 Published:2007-08-25
Contact: Xiang-jun Zeng

摘要/Abstract

摘要： 目的观察apelin-13对离体大鼠缺血/再灌注损伤的影响,并初步探讨其对apelin受体APJ(putative receptor protein related to the angiotensin receptor AT1)及细胞信号Akt1/2的影响?方法Langendorff 装置恒流灌注大鼠离体心脏，采用停灌/复灌方式复制缺血/再灌注模型；观察缺血/再灌注期间心脏收缩期左心室内压上升的最大变化速率（+ LVdp/dtmax）及舒张期左心室内压下降的最大变化速率（- LVdp/dtmax）；RT-PCR和Western blot测定组织中APJ受体mRNA和Akt1/2蛋白的表达情况。结果Apelin-13可以增加缺血/再灌注损伤心脏的±dp/dtmax（p<0.01）；可以明显增强心肌组织中Akt1/2的表达；缺血/再灌注可以引起心肌组织中APJ受体表达上调。结论Apelin-13拮抗缺血/再灌注引起的心脏收缩及舒张功能障碍；可能与组织中APJ受体表达上调引起Akt1/2表达增强有关。

关键词: 缺血/再灌注损伤, 心肌, apelin多肽

Abstract: Objective:To observe the protective effects of apelin-13 on on the expression of APJ (putative receptor protein related to the angiotensin receptor AT1)and Akt1/2 in ischemia/reperfusion injury rat heart in virtro Methods Langendorff device was used to perfuse isolated rat heart with constant flow. The ischemia/reperfusion models of rat heart in vitro were made through stopping and reperfusion of the KH fluid to the heart；observing the change of maximal rate of the pressure increase(+dp/dtmax) and maximal rate of the pressure decrease(-dp/dtmax) of left ventricle in rat heart . RT-PCR were used to assay the expression of APJmRNA in rat heart, Western blotting were used to assay Akt1/2 protein in rat heart. Results The groups given apelin-13 had higher ±LVdp /dtmax（p<0.01）; and resulted in hyper-expression of Akt1/2 protein (p<0.05), while the expression of APJmRNA were up regulated in IR group(p<0.05). Conclusion: Apelin-13 can resist the functional impairment in rat heart caused by ischemia/reperfusion injury, and the protecting effects of apelin-13 may involved in up regulated expression of APJ in rat heart, and the APJ might cause up regulated expression of Akt1/2 which would do good to I/R injury heart.

Key words: ischemia/reperfusion injury, Myocardium, apelin

曾翔俊王红霞芦玲巧王晓燕马利权张立克唐朝枢. Apelin-13对离体缺血/再灌注大鼠心脏损伤的影响[J]. 基础医学与临床, 2007, 27(8): 864-867.

Xiang-jun Zeng Hong-xia Wang Ling-qiao Lu Xiao-yan Wang Li-quan Ma Li-ke Zhang Chao-shu Tang. Protective effects of apelin-13 on the ischemia/reperfusion injury in rat heart[J]. Basic & Clinical Medicine, 2007, 27(8): 864-867.

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