中药筋脉通可通过p38 MAPK通路促进高糖条件下雪旺细胞nMSC表型向MSC表型分化

doi:10.16352/j.issn.1001-6325.2026.11.1519

基础医学与临床 ›› 2026, Vol. 46 ›› Issue (11): 1519-1525.doi: 10.16352/j.issn.1001-6325.2026.11.1519

中药筋脉通可通过p38 MAPK通路促进高糖条件下雪旺细胞nMSC表型向MSC表型分化

石玥¹, 梁晓春^1*, 孙青¹, 王晓婉²

1.中国医学科学院北京协和医学院北京协和医院中医科,北京 100730;
2.中国医学科学院北京协和医学院基础医学研究所细胞资源中心,北京 100005

收稿日期:2026-05-26 修回日期:2026-09-08 出版日期:2026-11-05 发布日期:2026-10-26
通讯作者: ^*xcliang@vip.sina.com
基金资助:
国家自然科学基金青年科学基金(81703908)

Traditional Chinese medicine Jin-Mai-Tong promotes differentiation of nMSC phenotype to MSC phenotype in Schwann cells under high-glucose conditions through p38 MAPK pathway

SHI Yue¹, LIANG Xiaochun^1*, SUN Qing¹, WANG Xiaowan²

1. Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730;
2. Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China

Received:2026-05-26 Revised:2026-09-08 Online:2026-11-05 Published:2026-10-26
Contact: ^*xcliang@vip.sina.com

摘要/Abstract

摘要： 目的探究中药筋脉通(JMT)对高糖条件下雪旺细胞的分化影响。方法原代培养雪旺细胞(SCs),建立高糖损伤模型,用JMT干预培养,对SCs中髓鞘形成表型SCs(MSCs)细胞进行计数;通过ELISA测定培养上清液中脑源性神经营养因子(BDNF)、胶质细胞源性神经营养因子(GDNF)蛋白表达;测定p38丝裂原活化蛋白激酶(p38 MAPK)、c-jun、EGR2蛋白及mRNA的表达。结果在高糖条件下存在p38 MAPK通路激活,c-JUN蛋白表达增加、EGR2表达下降(P＜0.01),而JMT提取物可显著抑制p38 MAPK通路激活,抑制c-JUN蛋白表达、提高EGR2表达,增加BDNF、GDNF蛋白分泌(P＜0.05)。结论 JMT可通过抑制p38 MAPK通路,提高MSCs分化,增加神经营养因子的合成。

关键词: 筋脉通, 雪旺细胞, p38 MAPK 通路, 髓鞘形成表型

Abstract: Objective To investigate the effect of the Chinese herbal medicine Jin-Mai-Tong (JMT) on the differentiation of Schwann cells (SCs) under high-glucose conditions. Methods Primary Schwann cells were cultured to establish a high-glucose injury model, and intervened with JMT. The number of myelinating phenotype Schwann cells(MSCs) was counted; The protein expression levels of brain-derived neurotrophic factor(BDNF) and glial cell line-derived neurotrophic factor(GDNF) in culture supernatant were determined via ELISA; The expression levels of p38 mitogen-activated protein kinase(p38 MAPK), c-JUN, early growth response protein 2(EGR2) at both protein and mRNA levels were detected. Results The p38 MAPK pathway was activated under high-glucose conditions, accompanied by up regulated c-JUN protein expression and down regulated EGR2 expression. JMT extract significantly inhibited the activation of the p38 MAPK pathway, suppressed c-JUN protein expression, up regulated EGR2 expression, and increased the secretion of BDNF and GDNF proteins. Conclusions JMT can promote the differentiation of MSCs and increase the synthesis of neurotrophic factors by inhibiting the p38 MAPK pathway.

Key words: Jin-Mai-Tong, Schwann cell, p38 MAPK pathway, myelinating phenotype

中图分类号:

R285.5

石玥, 梁晓春, 孙青, 王晓婉. 中药筋脉通可通过p38 MAPK通路促进高糖条件下雪旺细胞nMSC表型向MSC表型分化[J]. 基础医学与临床, 2026, 46(11): 1519-1525.

SHI Yue, LIANG Xiaochun, SUN Qing, WANG Xiaowan. Traditional Chinese medicine Jin-Mai-Tong promotes differentiation of nMSC phenotype to MSC phenotype in Schwann cells under high-glucose conditions through p38 MAPK pathway[J]. Basic & Clinical Medicine, 2026, 46(11): 1519-1525.

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中药筋脉通可通过p38 MAPK通路促进高糖条件下雪旺细胞nMSC表型向MSC表型分化

Traditional Chinese medicine Jin-Mai-Tong promotes differentiation of nMSC phenotype to MSC phenotype in Schwann cells under high-glucose conditions through p38 MAPK pathway

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