假基因AC106872.1参与维持人类胚胎干细胞自我更新能力

doi:10.16352/j.issn.1001-6325.2025.05.0561

基础医学与临床 ›› 2025, Vol. 45 ›› Issue (5): 561-567.doi: 10.16352/j.issn.1001-6325.2025.05.0561

假基因AC106872.1参与维持人类胚胎干细胞自我更新能力

蒋正阳, 孙梦瑶, 何浏, 余佳^*, 马艳妮^*

中国医学科学院基础医学研究所北京协和医学院基础学院生物化学与分子生物学系,北京 100005

收稿日期:2025-02-13 修回日期:2025-03-18 出版日期:2025-05-05 发布日期:2025-04-23
通讯作者: ^* mayanni@ibms.cams.cn;j-yu@ibms.pumc.edu.cn
基金资助:
国家自然科学基金(32470847)

Pseudogene AC106872.1 is involved in maintaining the self-renewal capacity of human embryonic stem cells

JIANG Zhengyang, SUN Mengyao, HE Liu, YU Jia^*, MA Yanni^*

Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005, China

Received:2025-02-13 Revised:2025-03-18 Online:2025-05-05 Published:2025-04-23
Contact: ^* mayanni@ibms.cams.cn;j-yu@ibms.pumc.edu.cn

摘要/Abstract

摘要： 目的探究假基因AC106872.1对维持人类胚胎干细胞(hESCs)自我更新能力的影响。方法在hESCs中敲除假基因AC106872.1,通过PCR及琼脂糖凝胶电泳检测敲除情况;通过集落形成实验及碱性磷酸酶(AP)染色评估hESCs的集落形成能力,qPCR及流式细胞测量术分析检测hESCs的多能性及分化标志基因表达情况,高通量测序检测敲除假基因对细胞转录组的影响。结果敲除假基因AC106872.1后,hESCs的集落形成能力得到抑制(P＜0.05),多能性标志基因表达水平显著降低(P＜0.000 1),分化标志基因表达水平显著上升(P＜0.000 1)。结论假基因AC106872.1通过调控多能性基因的表达,参与人类胚胎干细胞自我更新能力的维持。

关键词: 假基因, 人类胚胎干细胞, 多能性

Abstract: Objective To explore the role of pseudogene AC106872.1 in maintaining the self-renewal capacity of human embryonic stem cells (hESCs). Methods AC106872.1 was knocked out in hESCs and knockout efficiency was validated by PCR and agarose gel electrophoresis. The colony formation of hESCs was assessed through colony formation assays and alkaline phosphatase (AP) staining. The expression level of pluripotency and differentiation marker genes was analyzed by qPCR and flow cytometry. RNA sequencing (RNA-seq) was performed to assess transcriptomic changes upon AC106872.1 knockout. Results Knockout of AC106872.1significantly inhibited the colony formation of hESCs (P＜0.05). The expression level of pluripotency marker genes was significantly reduced (P＜0.000 1), while the expression of differentiation marker genes was markedly increased (P＜0.000 1). Conclusions The pseudogene AC106872.1 plays a crucial role in maintaining human embryonic stem cell self-renewal through regulation of pluripotency genes expression.

Key words: pseudogene, human embryonic stem cells, pluripotency

中图分类号:

Q343.6

蒋正阳, 孙梦瑶, 何浏, 余佳, 马艳妮. 假基因AC106872.1参与维持人类胚胎干细胞自我更新能力[J]. 基础医学与临床, 2025, 45(5): 561-567.

JIANG Zhengyang, SUN Mengyao, HE Liu, YU Jia, MA Yanni. Pseudogene AC106872.1 is involved in maintaining the self-renewal capacity of human embryonic stem cells[J]. Basic & Clinical Medicine, 2025, 45(5): 561-567.

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假基因AC106872.1参与维持人类胚胎干细胞自我更新能力

Pseudogene AC106872.1 is involved in maintaining the self-renewal capacity of human embryonic stem cells

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