miR-199a-5p alleviates irradiation-induced injury in human umbilical vein endothelial cell line

doi:10.16352/j.issn.1001-6325.2026.11.1455

Abstract

Abstract: Objective To investigate the protective effect of miR-199a-5p on irradiation-induced injury in human umbilical vein endothelial cells (HUVECs) as well as underlying mechanism. Methods In order to determine the optimal irradiation dose for establishing the injury model.HUVECs were irradiated with X-rays of varying doses (3, 6, 9, and 12 Gy). Cell viability was assessed by CCK-8 assay. Apoptosis was examined by annexin V/PI double staining. The level of intracellular reactive oxygen species (ROS) was evaluated by dithydroethidium (DHE) fluorescent probe. The cells were divided into following four groups as control (Ctrl), irradiation alone (Ir), irradiation + negative control transfection (Ir+NC), and irradiation + miR-199a-5p mimic (Ir+miR-199a-5p). The effects of miR-199a-5p on apoptosis and ROS level were examined. Mitochondrial super oxide production and mitochondrial membrane potential (ΔΨm) were measured by MitoSOX^TM Red and JC-1 probes, respectively. Results Irradiation at 9 Gy significantly suppressed HUVECs viability (P＜0.05),induced apoptosis and enhanced intracellular ROS level(P＜0.05). Over-expression of miR-199a-5p markedly attenuated irradiation-induced apoptosis and oxidative stress (P＜0.05). Furthermore, miR-199a-5p effectively suppressed mitochondrial super oxide generation (P＜0.05) and maintained mitochondrial membrane potential stability after irradiation. Conclusions miR-199a-5p may alleviate irradiation-induced HUVECs injury by inhibiting mitochondrial oxidative stress and stabilizing mitochondrial membrane potential.

Key words: miR-199a-5p, irradiation injury, mitochondrial, oxidative stress, apoptosis

CLC Number:

R541.9

LIU Chenxi, WEN Wen, QU Mingyue, JIN Zhitao, LU Xiaojiong, XU Jiaojiao, ZHANG Zheng. miR-199a-5p alleviates irradiation-induced injury in human umbilical vein endothelial cell line[J]. Basic & Clinical Medicine, 2026, 46(11): 1455-1462.

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