Abstract: Objective To investigate the effect of SEMA3G protein overexpression on proliferation, invasion and migration of human pancreatic cancer PANC-1 cells in vitro. Methods PANC-1 cells of human pancreatic cancer were transfected with lentiviral vector carrying SEMA3G. The expression of SEMA3G at mRNA and protein level in celsl were detected by real-time PCR and western blot, respectively. CCK8 assay was applied to examine cell proliferation, the cell invasion was analyzed by transwell assay and wound healing assay was used to examine cell migration in PANC-1 cells. Results The results showed that stable transfected SEMA3G cell line of pancreatic cancer line was established successfully. The proliferation capacity of SEMA3G group was significantly lower compared to blank and the negative control groups (P<0.05). Similarly, cell invasion and migration capacity of SEMA3G group were significantly lower than the two kinds of control groups(P<0.05). Conclusions SEMA3G lentiviral vectors can effectively increase SEMA3G protein expression in PANC-1 cells, thereby inhibiting cell proliferation, invasion and migration.

Key words: lentiviral, pancreatic cancer, cell proliferation, migration