Abstract: Objective To investigate the etiological role of human papillomavirus(HPV) infection in the carcinogenesis of non-small cell lung cancer(NSCLC) and to discuss the possible relationship between HPV infection and HIF-1α,VEGF expression. Methods By using immunohistochemistry, the expression of HIF-1α and VEGF protein was detected in 60 cases of NSCLC tissues and 20 cases of lung benign diseases tissues. PCR was employed to detect the expression of HPV16/18 DNA. Results ① The HPV DNA expression was 0.6046±0.0224 in NSCLC group significantly higher than 0.0275±0.1230 in the benign diseases group (P<0.05). ②The HIF-1α protein and VEGF protein expression was 0.2548±0.0219 and 0.2051±0.0321 respectively in NSCLC, significantly higher than in the benign diseases group (0.0826±0.0150 and 0.0726±0.0227) (P<0.05). ③The expression rate of HIF-1α was significantly correlated with VEGF in NSCLC（P<0.05）.④The positive expression rate of VEGF was significantly higher in HPV DNA positive group(56.0%)than in HPV DNA negative group(28.6%,P<0.05) in NSCLC.Conclusion HPV infection may be one of the etiological factors in the carcinogenesis of NSCLC. HPV infection increased VEGF expression, promoted the occurrence and development of NSCLC.

Key words: non-small cell lung cancer, human papillomavirus, hypoxia inducible factor-1α, vascular endothelial growth factor