Abstract: Objective The localization of HSD13 in tissues and its effect on cell cycle were investigated. Methods HSD13 was acquired by electronic cloning method. The expression of HSD13 in different tissues was detected by Northern blot and Western blot. The localization of HSD13 in testis was observed by immunohistochemistry. Double thymidine block method in combination with flow cytometry was used to study the effect of HSD13 overexpression on cell cycle. Results The sequence of HSD13 was obtained by electronic cloning method. HSD13 was highly expressed in testis, and was mainly located in spermatogonia and primary spermatocytes. HSD13 overexpression and control CHO stable cell lines were constructed. Overexpression of HSD13 could significantly accelerate G2/M phase process in cell cycle. Conclusion HSD13 was highly expressed in spermatogonia and primary spermatocytes of testis, and its overexpression could accelerate cell cycle progression.

Key words: HSD13, spermatogenesis, cell cycle, FACS