Abstract: Objective To investigate the effect of NF-κB inhibitor—pyrrolidine dithiocarbamat (PDTC) on the expression of apoM in renal cortex of rats with ischemia reperfusion injury (IRI) . Methods Seventy-five male rats were randomly divided into sham operation group、IRI group and PDTC group，five subgroups at time points of 3，6，12，24 and 48h after reperfusion were set up in each group. The expression of mRNA and protein of apoM in cytoplasm and that of NF-κB P65 protein in nucleus of renal cortex were detected at the indicated time points. Results The expression of NF- кBp65 protein in IRI group was obviously higher than that in sham operation group（sham：1.0±0.2；IRI3h：4.1±0.5，P＜0.01）, while the expression in PDTC group was lower than that in IRI group（PDTC3h：2.0±0.2，P＜0.01）, and two peaks were detected, which were respectively at 3 and 48h after reperfusion. A similar expression pattern of both mRNA and protein of apoM was found among the three groups, and there were also two peaks in expression of apoM, the expression peaks of apoM mRNA was at 3 and 48h after reperfusion, while those of apoM protein lag behind. Otherwise, a significant correlation was found between the expression of apoM mRNA and NF--κB P65. Conclusion The results indicate that apoM feasibly takes part in the pathogenesis of IRI via NF-кB.

Key words: kidney, Ischemia reperfusion, Apolipoprotein M, NF-kappa B