Abstract: Objective To study Adenosine inhibits lipopolysaccharide-induced human umbilical vein endothelial cell (HUVEC) apoptosis. Methods The cultured HUVEC was divided into 6 groups: Control group, LPS group, dimethylsulfoxide (DMSO) group, Low, Medium and High-dose adenosine group. the cell's shape structure were observed continuously by fluorescence microscope. TNF-α expression in the supernate was determined by ELISA. Cells's apoptosis was examined by Flow Cytometry. Results The TNF-α expression and cell apoptosis were significantly increased (p＜0.05); Groups of different doses of adenosine can inhibit the expression of TNF-α and cell apoptosis (p＜0.05). Conclusion Certain dose of adenosine can inhibits lipopolysaccharide-induced the HUVEC release inflammation medium, adenosine can also suppress cell apoptosis.

Key words: Adenosine, LPS, HUVEC, Inflammation, Apoptosis