Abstract: Objective To explore the role of PI3K/AKT signaling pathway in total hepatic ischemia -reperfusion (I/R)-induced lung injury in rats. Methods This study was divided into 2 parts.（1）36 rats were killed respectively at preischemia and different time after reperfusion,and the lung tissue was taken.(2)12 rats were randomly divided into Wortmannin group and model control group. AKT,p-AKT protein expression,apoptotic cells and PCNA protein expression was determined respectively by Western blot,TUNEL and immunohistochemistry analysis.Results （1）Compared with that in preiscemia group,after I/R the apoptotic index (AI)was increased,the p-AKT/AKT ratio and PCNA - positive index showed bidirectional changes，and the histological changes was severe.（2）p-AKT/AKT ratio showed a positive correlation with PCNA-positive index and a negative correlation with AI .（3）Compared with that in model control group, the p-AKT/AKT ratio and PCNA-positive index was lower, histological changes was more severe and AI was higher in Wortmannin group. Conclusion PI3K/AKT signaling pathway had protective effect on lung injury induced by total hepatic I/R ,which was maybe mediated by anti-apoptosis and promoting proliferation.

Key words: PI3K/AKT signaling pathway, Ischemia-reperfusion, Lung injury