Abstract: Objective To study the Extracelluar Regulated protein Kinase(ERK) phosphorylation in gastric mucosal cell by the serum derived from the rats treated with electroacupuncture at stomach meridian acupoints. Methods 72 rats were randomly divided into Normal group, Model group, Stomach group, Gallbladder group, Stomach plus PD153035 group and Gallbladder plus PD153035 group, gastric mucosal cells were separated by using pronase digestion method, and incubated by PD153035, a EGFR inhibitor and 100ml/L serum in sequence, ERK phosphorylation in gastric mucosal cells was tested by Western blot. Results In both Stomach group and Gallbladder group, compared with the Normal group or Model group, the ERK phosphorylation in gastric mucosal cell were more reinforcement（P<0.01）. Furthermore, the effects in Stomach group were more obvious than those in Gallbladder group（P<0.01）. Compared with Stomach group, the reduction of the ERK phosphorylation in Stomach plus PD153035 group was more significant（P<0.01）.Conclusions The serum derived from the rats treated with electroacupuncture at stomach meridian acupoints can activate the ERK phosphorylation in gastric mucosal cells, which also could be one proof for the TCM theory "Relative particularity between Meridian and Viscera".

Key words: electroacupuncture, serum, gastric mucosal cell, ERK, phosphorylation