Abstract: Objective To investigate the effects of bone marrow mesenchymal stem cells administration on expression of VEGF、SDF-1、TGF-β1 and heart function in myocardial infarcts rats. Methods MSCs were harvested from SD rats, collected from bone marrow, cultured and passaged in vitro, then purified by density gradient centrifugation and adhesive-screening method. Rats were randomly divided into 2 groups. In the control group (n =8) were not treated with stem cells as the experiment group, but saline of the same volume. Four weeks after the injection,the expression of VEGF、SDF-1、TGF-β1 and heart function were examined. Results Four weeks after the transplantation，Immunohistochemistry showed expression of VEGF and SDF-1 increased markedly in experiment group,and expression of TGF-β1 decreased. Heart function was improved in the experiment group(P<0.05). Conclusion MSCs transplantation can interfere in the secretion of VEGF、SDF-1、TGF-β1 and improve the heart function.

Key words: stem cells, cytokine, myocardial infarcts, transplantation