Abstract: Objective To study the differential proteome of kidney between lupus nephritis mouse and normal mouse. Methods The proteins of kidney were separated by two-dimensional gel electrophoresis(2-DE). The gels stained by silver were scanned by ImageScanner and analyzed by PDQuest software. Results The experiments were repeated for three times. About 573±52 and 658±43 protein spots were found in the three maps of control group and LN group respectively; the match ratio is 83% and 87% respectively. Analyzed by PDQuest software,114 spots were found increased for two folds through comparing LN group to control group,and 140 spots were found decrease for 0.5 folds. Conclusions A significant differences in protein expression of LN mouse kidney,which may be related to the etiopathogenesis of LN,had been found.

Key words: lupus nephritis, systemic lupus erythematosus, kidney, proteome, two dimensional electrophoresis