Abstract: Abstract: Objective To study expression changes of Ephrin and its receptors in DRG neurons after peripheral nerve injury. Methods Set up animal model of unilateral crushed sciatic nerve, and by immunostaining screened the expression pattern changes of Ephrin B1, Eph B1、Eph B2、Eph B3、EphA4 and Ryk in DRG neurons after unilateral sciatic nerve crushed, quantified the positive staining cells in DRG and analyzed the percentage of positive cells in different size. Results After peripheral nerve injury, the expression of Ephrin B1 significantly decrease, at the same time there were no any expression change of Eph B1、Eph B2、Eph B3 or Eph A4, but the Ryk expression was intensely induced in DRG neurons. Conclusion Because the Ephrin B1 and Ryk show very strong expression changes in DRG neurons after peripheral nerve injury, so this result strongly suggests they might involved in the process of peripheral never regeneration.

Key words: Dorsal root ganglion, Sciatic Nerve, Injury, Ephrin B1, Ryk