Abstract: Abstract : Objective: The objective was to look　for cocaine-and amphetamine-regulated　transcript peptide(CART) receptors or proteins interacted to CART. Method :A cDNA library from rat brain was constructed. Then the BacterioMatch two-hybrid system was used to screening for proteins interacted with CART by using CART41-102 as bait. Result:The cDNA library and bait pBT- CART41-102 were constructed successfully. The library harvested 3.37×106 individuals, and the reconstruction rate was 98.5%. 5.01x106 cotransformed clones were screened, 93 clones were conformtively identified to interacting with CART and were DNA sequenced. By bioinformation analysis, 6 known proteins interacted to CART were identified. Other unknown interaction proteins that had short exactly similiar motif to 22 membraneproteins or receptors were also identified. Conclusion: CART might interact to proteins about energy balance, cell proliferation and enzymes related to protein processing and degrading .

Key words: cDNA library, CART, bacterial two-hybrid system, protein-protein interaction