关键词: 重组腺病毒, 肾脏, 基因, 大鼠

Abstract: Objective To investigate expression of recombinant adenovirus(rAV) vector-mediated report gene in kidney, and seek for the best way of rAV vector-mediated gene transfer in vivo. Methods Thirty Sprague-Dawley(SD) rats were divided into 5 groups randomly. rAV－GFP(1013viral particales·L-1 ) was injected through different ways. 2, 7 and 14d after injection, the GFP expression in left kidney, right kidney, liver and lung was evaluated by fluorescence microscope and computer software. Results GFP gene expressed in kidey and 7d after injection expression reach peak, through intraperitoneal and caudal vein injection expressions were most; through renal artery has the best target. Conclusion rAV vector-mediated report gene could be transducted in kidney. The rAV-mediated transgene expression in kidney is a potential strategy in treatment kidney diseases and different ways have their own characteristics.

Key words: recombinant adenovirus, kidney, gene, rat