rL-RVG抑制人小细胞肺癌细胞系NCI-H446的增殖及迁移

doi:10.16352/j.issn.1001-6325.2022.10.1549

基础医学与临床 ›› 2022, Vol. 42 ›› Issue (10): 1549-1554.doi: 10.16352/j.issn.1001-6325.2022.10.1549

rL-RVG抑制人小细胞肺癌细胞系NCI-H446的增殖及迁移

梁冰¹, 严玉兰^2*

1.昆山市中医医院呼吸科,江苏昆山 215300;
2.江苏大学附属人民医院呼吸科,江苏镇江 212000

收稿日期:2021-05-28 修回日期:2022-01-07 出版日期:2022-10-05 发布日期:2022-09-23
通讯作者: * ylyan2005@163.com
基金资助:
苏州市科技发展计划(民生科技)(SYS2020066);昆山市社会发展项目(KS1653)

rL-RVG inhibits the proliferation and migration of human small cell lung cancer cell line NCI-H446

LIANG Bing¹, YAN Yu-lan^2*

1. Department of Respiratory Medicine, Kunshan Hospital of Traditional Chinese Medicine, Kunshan 215300;
2. Department of Respiratory Medicine, the Affiliated People's Hospital of Jiangsu University, Zhenjiang 212000, China

Received:2021-05-28 Revised:2022-01-07 Online:2022-10-05 Published:2022-09-23
Contact: * ylyan2005@163.com

摘要/Abstract

摘要： 目的探讨重组新城疫病毒rL-RVG对人小细胞肺癌细胞系NCI-H446增殖、迁移的影响及可能的作用机制。方法将NCI-H446细胞随机分为3组,分别加入PBS、新城疫病毒(NDV)和rL-RVG,PBS为对照组。用免疫荧光法检测RVG及NDV蛋白在细胞内的表达;用MTT法测定病毒的抗增殖情况;划痕实验及Transwell小室法检测细胞迁移情况;免疫荧光法检测相关迁移蛋白E-cadherin和MMP2的表达。结果 rL-RVG在NCI-H446细胞中的感染率高,并能够在其内稳定表达;rL-RVG对小细胞肺癌细胞的抑制效果强于NDV。rL-RVG组划痕距离显著小于NDV组及对照组(P＜ 0.05);rL-RVG组穿过小室的细胞数明显少于对照组(P＜ 0.05)。相较于NDV及对照组,rL-RVG组的MMP2蛋白表达下降,而E-cadherin蛋白表达上调。结论 rL-RVG能够抑制NCI-H446细胞增殖,其机制可能是通过调节E-cadherin和MMP2而影响细胞迁移。

关键词: 小细胞肺癌, 新城疫病毒, 迁移

Abstract: Objective To investigate the effect and potential mechanism of recombinant Newcastle disease virus rL-RVG on proliferation and migration of human small cell lung cancer cell line NCI-H446. Methods NCI-H446 cells were divided into 3 groups: incubated with PBS, Newcastle disease virus(NDV) and rL-RVG, respectively. Immunofluorescence assay was used to detect the expression of RVG and NDV protein. MTT assay was used to determine the anti-proliferation of the virus. The cell migration was detected by scratch test and Transwell method. Immuno-fluorescence assay was used to detect the expression of E-cadherin and MMP2 proteins. Results After infection, rL-RVG stably expressed in NCI-H446 cell with high infection efficiency.The scratch distance in rL-RVG group was significantly shorter than that in NDV group and control group(P＜0.05). In the Transwell experiment, the number of cells crossing the compartment in the rL-RVG group were significantly less than that in the control group(P＜0.05). MMP2 protein expression was down-regulated in rL-RVG group, while E-cadherin protein expression was up-regulated(P＜0.05), compared with NDV group and control group. Conclusions rL-RVG could inhibit the proliferation of NCI-H446 cell with the potential mechanism of effects on cell migration through regulating E-cadherin and MMP2.

Key words: small cell lung cancer, Newcastle disease virus, migration

中图分类号:

梁冰, 严玉兰. rL-RVG抑制人小细胞肺癌细胞系NCI-H446的增殖及迁移[J]. 基础医学与临床, 2022, 42(10): 1549-1554.

LIANG Bing, YAN Yu-lan. rL-RVG inhibits the proliferation and migration of human small cell lung cancer cell line NCI-H446[J]. Basic & Clinical Medicine, 2022, 42(10): 1549-1554.

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rL-RVG抑制人小细胞肺癌细胞系NCI-H446的增殖及迁移

rL-RVG inhibits the proliferation and migration of human small cell lung cancer cell line NCI-H446

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