Abstract: Objective To investigate ASPS induced G2/M arrest in small cell lung cancer cell lines H446 and its effect on ERK MAP kinase signal transduction pathways. Methods Cell cycle phases were inspected by flow cytometery (FCM) ; Western blot analysis inspected the proteins of ERK, p-ERK; Results Compared with control group, G2/M phase cells increased with concentration increase significantly, G0/G1 phase cells were no difference, G2/M phase cells and G0/G1 phase cells were no difference when pre-incubated with PD98059 prior to exposure to ASPS of different concentrations, proteins of p-ERK were increased significantly, expression of ERK were no difference. Conclusion ASPS may induce G2/M arrest of H446 cells possibly by activation ERK MAP kinase pathways.

Key words: Acanthopanax senticosus polysaccharide, small cell lung cancer cell lines (H446), G2/M arrest, ERK, P38