Abstract: Objective: We aimed to study the influence of miR-205 on the normal human epidermal keratinocytes (NHEKs) migration by using synthesized antagomir-205 and recombinant lentivirus expressing miR-205, respectively. Methods: Cultured NHEKs were treated with Synthesized antagomir-205. Northern blot analysis was conducted to detect whether endogenous level of miR-205 could be reduced by antagomir-205. Wound healing assay was adopted to further measure the cell migratory ability of NHEKs. Meantime, we also tested its influence on NHEKs by wound healing assay after miR-205 recombinant lentivirus infecting NHEKs. Results: The migratary ability of NHEKs was significantly inhibited by antagomir-205, while ectopic expression of miR-205 promoted NHEKs to migrate. Conclusion: Ectopic expression of miR-205 promoted the normal human epidermal keratinocytes to migrate.

Key words: microRNA, epithelial cells, cell migration