Abstract: 【Abstract】 Objective To study the mechnism of toxicity by cisplatin on RPTC cell line and Bcl-2 anti-apoptosis mechanism with transfection of different mutant Bcl-2 in vitro. Methods RPTC cell was translocated with different mutant Bcl-2(s). The cell apoptosis induced by cisplatin on RPTC ce11 were analyzed with confocal and flurencent microscope.the cell apoptosis was measured with Hoechst33258 after treated with cisplatin. Results Different mutant Bcl-2（Bcl-acta，Bcl-cb5）were translocated on mitochondrial and Endoplasmic Reticulum(ER) respectively.Bcl-acta can protect RPTC cell from apoptosis easily more than Bcl-cb5 group in time-dependant manner by cisplatin treated (P<0.05). Conclusion Cisplatin can induced RPTC cell apoptosis obivously by mitochondrial signaling payhway and Bcl-2 have protect more RPTC cell from apoptosis in mitochondrial manner other than in ER.

Key words: Cell apoptosis, Cisplatin, Bcl-2 gene, Signaling transduction