Abstract: Objective To study the relationship of activated PAK1 (pPAK1Thr423) with the subcellular localization and protein level maintenance of survivin in the meiotic progression of mouse oocytes. Methods Immunofluorescent staining was employed to analyze the spatial-temporal correlation between pPAK1Thr423 and survivin during oocyte meiosis; Western blot was applied to analyze the change of survivin level in oocytes treated with PF3758309. Results In mouse oocytes at metaphase I stage (MI), pPAK1Thr423 was co-localized with survivin between chromosome arms and on centromeres of homologous chromosomes, the centromeric co-lozalization remained at metaphase II stage (MII), while that across chromosome axis was confined in the local space between sister centromeres. The protein level of survivin was significantly decreased in oocytes treated with PF3758309. Conclusions pPAK1Thr423 may regulate the subcellular localization and protein maintenance of survivin, so as to participate in CPC regulating chromosome separation in mouse oocyte meiosis.

Key words: mouse, oocyte, meiosis, pPAK1Thr423, survivin