Abstract: Objective To investigate the proliferation and invasion in human pancreatic cancer after Stat1 gene silenced or enhanced and to explore the possible underlying mechanisms．Methods BxPC-3 cells were divided to three groups: Stat1 group which transfect with specific Stat1 plasmid, Si Stat1 group which transfected with specific Stat1 small interfering RNA (siRNA), and control group. Western blot was used to detect the expression of Stat1, VEGF, MMP-2 and MMP-9 in BxPC-3 cells. MTT method was used to detect the proliferation of BxPC-3 cells. Transwell was used to detect the invasion of BxPC-3 cells．Results The proliferation and invasion were significantly enhanced in Pancreatic Cancer Cells after Stat1 gene silenced, and the expression of VEGF, MMP-2 and MMP-9 were increased. The proliferation and invasion were significantly decreased in Pancreatic Cancer Cells after Stat1 gene enhanced． Conclusion Stat1 inhibits the proliferation and invasion of pancreatic cancer. VEGF，MMP-2 and MMP-9 possibly play a role in this process.

Key words: pancreatic cancer, BxPC-3, Stat1, siRNA, plasmid, VEGF, MMP-2, MMP-9