Abstract: Objective To investigate the effects of adiponectin(ADPN) on cell proliferation and the oxidative stress level of INS-1 cultured with high concentration of D-glucose and to illuminate the potential protection of ADPN on the INS-1.Methods The INS-1 cells culture in vitro ：The INS-1 cells were cultured with 5.5mmol/L glucose(control group), 25.6 mmol/L glucose(high glucose group), 25.6mmol/L glucose and concentration of 2.5mg/l adiponectin(ADPN group) , 25.6mmol/L glucose and concentration of 10.0μmol/L SB203580(SB203580 group) in random.After culture for 24,48,72h, the proliferation activity of INS-1 was observed by CCK-8.The content of MDA and T-AOC of the cell supernatant was measured by colorimetry. The expression of t-P38MAPK, p-P38MAPK were examined by Western blot.Results Compared with control group, INS-1 proliferation activity, the activation of P38MAPK, the content of T-AOC and MDA, have difference in high glucose group in 24,48,72h (P<0.05), and adiponectin or SB203580 could inhibit inhibit these changes(P<0.05). Conclusion Adiponectin could promote the proliferation of the INS-1,ameliorate the oxidative stress level and protect them in D-glucose of high concentration through the P38MAPK signaling pathway.

Key words: adiponectin, INS-1, P38MAPK, Proliferation, Oxidative stress