Analysis of Differential Urinary Proteome in Experimental Minimal Change Nephropathy
Yan WANG, You-he GAO
School of Basic Medicine Peking Union Medical College, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences Department of Physiology and Pathophysiology, School of Basic Medicine Peking Union Medical College, Institute of Basic Medical Sciences Chinese Academy of Medical Sciences
Objective To screen early urine protein markers for minimal change nephropathy. Method Adriamycin nephropathy was employed as minimal change nephropathy model. Urinary protein and ConA captured glycoproteins were respectively profiled. Result By profiling urine proteome, 25 differential proteins were identified. These differential proteins were from leaked plasma proteins, secreted proteins from immuno- and in?ammatory cells, specific secreted proteins from urinary tract, and et al. They take part in different pathogenic process, eg. hemodynamic changes, podocytes injury, immunological disorder and et al. By profiling ConA-enriched urinary glycoproteome, 21 differential proteins were identified, among which 12（57%）were different from the above 25 differential proteins. This indicates that the knowledge of urine glycoproteome is complementary to urine proteome in understanding kidney condition. Conclusion These differential proteins can be potential indicators of minimal change nephropathy, and can help better understand the pathogenesis by further studying their functions.