葛根素减轻乙醇导致大鼠生精细胞的凋亡

基础医学与临床 ›› 2009, Vol. 29 ›› Issue (12): 1291-1295.

葛根素减轻乙醇导致大鼠生精细胞的凋亡

何红云邓仪昊杨新文杨开明

大理学院基础医学院四川大学基础医学与法医学院大理学院基础医学院

收稿日期:2009-01-06 修回日期:2009-04-29 出版日期:2009-12-20 发布日期:2009-12-20
通讯作者: 何红云

Puerarin Reduces Ethanol-induced-Apoptosis of Spermatogenic Cells in Rat Testis

Hong-yun HE, Yi-hao DENG, Xin-wen YANG, Kai-ming YANG

School of Preclinical,Dali University School of Preclinical and Forensic Medicine, Sichuan University

Received:2009-01-06 Revised:2009-04-29 Online:2009-12-20 Published:2009-12-20
Contact: Hong-yun HE,

摘要/Abstract

摘要： 目的观察乙醇导致大鼠生精细胞的凋亡及葛根素的干预。方法将大鼠30 只，随机均分为对照组、乙醇组及葛根素干预组。于实验第40天免疫组织化学法（SABC）检测左侧睾丸各组Bcl-2、Bax 蛋白在生精细胞的表达； RT-PCR检测右侧睾丸各组Bcl-2及Bax mRNA的表达；TUNEL 法检测生精细胞的凋亡。结果醇组平均每个生精小管断面中Bcl-2 蛋白阳性细胞数和A值低于对照组(P<0.01)，而平均每个生精小管断面中Bax 蛋白的阳性细胞数和A值高于对照组(P<0.01)；乙醇组Bax mRNA表达较葛根素干预组及对照组强（P<0.05），而Bcl-2 mRNA表达较葛根素干预组及对照组弱（P<0.05）；乙醇组每个生精小管横切面中的凋亡细胞数目高于对照组(P< 0.01),葛根素干预组显著缓解上述变化。结论根素对乙醇导致的大鼠生精细胞凋亡有干预作用。

关键词: 乙醇, 葛根素, 生精细胞, 凋亡

Abstract: 0bjective To investigate whether puerarin could protect against ethanol-induced apoptosis of spermatogenic cells in rat testis．Methods 30 S.D adult male rats，were randomly divided into three groups: normal control group ,alcohol group and puerarin group. At the 40 day， Bcl-2 and Bax of spermatogenic cells of testis tissue was checked by RT-PCR and immunohistochemistry; Apoptosis of spermatogenic cells was determined by TUNEL. Results The results of RT-PCR and immunohistochemistry indicated that Bcl-2 and Bax of spermatogenic cells were no significant statistical difference between group B and group C(P>0.05)，but there was significant statistical difference between group A and group B (P<0.01). Apoptosis of spermatogenic cells in group A were significantly higher than group C. Conclusion Spermatogenic cells could generate apoptosis by changing the expression of Bcl-2 and Bax. Puerarin could inhibit this damage of didymus by alcohol.

Key words: Ethanol, Spermatogenic cells, Puerarin, Apoptosis

何红云邓仪昊杨新文杨开明. 葛根素减轻乙醇导致大鼠生精细胞的凋亡[J]. 基础医学与临床, 2009, 29(12): 1291-1295.

Hong-yun HE; Yi-hao DENG; Xin-wen YANG; Kai-ming YANG. Puerarin Reduces Ethanol-induced-Apoptosis of Spermatogenic Cells in Rat Testis[J]. Basic & Clinical Medicine, 2009, 29(12): 1291-1295.

[1]	刘雯霞刘彩红郭蕊孟芝君高佳王亚静. 尼古丁促进高糖/高脂培养的大鼠心肌细胞系H9C2凋亡[J]. , 2020, 40(1): 24-29.
[2]	蒋庆锋于永魁程金华申思宁邢文群. lncRNA HEIH通过miR-98-5p调节肺癌细胞增殖和凋亡[J]. , 2020, 40(1): 30-36.
[3]	苗玉娟王淑坤孙丹丁康刘美逸张妍周风华. miR-181 通过 Prox1 促进人乳腺癌细胞系MCF-7的增殖并抑制凋亡[J]. 基础医学与临床, 2019, 39(9): 1265-1269.
[4]	童静凯江霞. 托伐普坦对小鼠内髓集合管细胞系水通道蛋白及肾小管功能的影响[J]. 基础医学与临床, 2019, 39(8): 1163-1167.
[5]	晋瑞金迎迎李嫚雷喆. 过表达脾酪氨酸激酶降低氧化应激诱导的小鼠成骨细胞凋亡[J]. 基础医学与临床, 2019, 39(7): 1013-1018.
[6]	任玮余宏川王萍刘英茹. miR-17-5p诱导小鼠肾足细胞系凋亡[J]. 基础医学与临床, 2019, 39(7): 973-977.
[7]	雷晓燕孙永红陈星星崔维静刘璟. 乙肝病毒X蛋白抑制小鼠足细胞系MPC5增殖并促进其凋亡[J]. 基础医学与临床, 2019, 39(5): 617-622.
[8]	马从乾王雅王娜杨柯陈德才. miR-181a过表达加重H2O2诱导的HUVECs损伤[J]. 基础医学与临床, 2019, 39(5): 690-695.
[9]	王圣凯胡苏琴吴玲吴杭菲郑靖宇蒋自培李剑敏. miR-138在乙醇诱导的大鼠心肌细胞系H9C2氧化应激中表达增加[J]. 基础医学与临床, 2019, 39(5): 677-681.
[10]	战仕胜李军杨婷婷王雅蓉王秀青. 抗菌肽merecidin诱导人肺腺癌细胞系A549凋亡[J]. 基础医学与临床, 2019, 39(4): 473-477.
[11]	刘莹张艳丽王秀芳. 沉默FOXO1抑制H2O2诱导的大鼠肺泡2型上皮细胞凋亡[J]. 基础医学与临床, 2019, 39(4): 519-523.
[12]	张娟冯燕和水祥. 过表达miR-5195-3p对结直肠癌细胞凋亡的影响[J]. 基础医学与临床, 2019, 39(3): 385-391.
[13]	张许梅王瑾霍海燕岳继萍张文婷焦向英. 血管紧张素II 1型受体自身抗体可上调TXNIP导致大鼠胰岛β细胞系INS-1凋亡[J]. , 2019, 39(11): 1548-1555.
[14]	宁娜侯晓鸿李杨何金玲燕子王晓晖王丽. 自噬降低激活β1-AA诱导的心肌细胞内质网应激相关凋亡途径[J]. 基础医学与临床, 2019, 39(1): 1-6.
[15]	唐静胡玲刘奉李小山赵梓亦李国利. 异氟醚抑制低氧/复氧诱导的肾小管上皮HK-2细胞系内质网应激凋亡[J]. 基础医学与临床, 2018, 38(9): 1258-1262.