基础医学与临床 ›› 2011, Vol. 31 ›› Issue (4): 422-425.

• 研究论文 • 上一篇    下一篇

蛋白激酶C(PKC)对人精子活动力的影响及其作用机制

丁玉芹,姜宏,汪存利   

  1. 安徽医科大学解放军临床学院生殖医学中心
  • 收稿日期:2010-07-05 修回日期:2010-10-13 出版日期:2011-04-05 发布日期:2011-04-08
  • 通讯作者: 姜宏 E-mail:jiangh105@sina.com

The effect of PKC on human sperm motility and its mechanism

Yu-qin DING1,Hong JIANG1,Cun-li WANG2   

  1. 1. Reproductive Medicine Center, the 105th Hospital of PLA,affiliated to Anhui Medical University
    2.
  • Received:2010-07-05 Revised:2010-10-13 Online:2011-04-05 Published:2011-04-08
  • Contact: Hong JIANG E-mail:jiangh105@sina.com

摘要: 目的 探讨人精子蛋白激酶C(PKC)的表达水平对精子活动力的影响及其作用机理。方法 收集30份正常精液(精子浓度≥20 × 106 / mL,a≥25%或a + b ≥50%)和20份弱精子症患者精液(精子浓度≥20 × 106 / mL,a+b≤40%)分别作为对照组和弱精症组。对照组先后加入PKC激活剂PMA和抑制剂GF109203X,用流式细胞术(FCM)检测精子中PKCβⅠ含量;用Western blot方法检测ERK蛋白和磷酸化表达水平。结果 弱精组精子PKCβⅠ含量显著低于对照组(P<0.05);加入PMA后精子ERK磷酸化水平明显升高(P<0.05),加入GF109203X后ERK磷酸化水平明显降低(P<0.05)。结论 精子活力低下的原因与PKC的含量降低有关,PKC可通过ERK信号转导途径参与调节精子活动力。

关键词: 精子活动力, 胞外信号调节激酶, 蛋白激酶C, 流式细胞术

Abstract: Objective To explore the effect of PKC on sperm motility and its mechanism. Methods After standard semen analysis, 30 healthy volunteers (sperm density ≥20×106 /ml, a≥25% or a + b ≥50% ) and 20 infertile males with asthenospermia (sperm density ≥20×106 /ml, a<25% and a+b≤40%) were classified as control group and asthenospermia group,and the spermatozoa PKCβⅠin two groups were measured by flow cytometry(FCM). Moreover, spermatozoa in control group were incubated with PMA (PKC activator)for 15min,then treated with GF109203X(PKC inhibitor)for 15min, and the phosphorylation and protein expression level of ERK were detected by Western blot. Results The level of PKCβⅠin asthenospermia group decreased significantly compared with control group. After incubation with PMA, following the improvement of sperm motility, the phosphorylation level of ERK in spermatozoa increased significantly, however, after incubation with GF109203X, both sperm motility and phosphorylation level of ERK decreased significantly. Conclusions PKC may involve in the regulation of sperm motility through ERK signal transduction pathway,and the level of PKC in spermatozoa may play an important role in pathogenesis of asthenospermia.

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