基础医学与临床 ›› 2011, Vol. 31 ›› Issue (4): 404-408.

• 研究论文 • 上一篇    下一篇

慢性粒细胞白血病肿瘤干细胞的免疫调节功能

王椋1,曲学彬1,2,赵春华1   

  1. 1. 中国医学科学院 基础医学研究所 北京协和医学院 组织工程中心
    2.
  • 收稿日期:2010-11-29 修回日期:2011-01-19 出版日期:2011-04-05 发布日期:2011-04-08
  • 通讯作者: 赵春华 E-mail:zhaoch16@hotmail.com

The immuno-modulatory defect of Cancer Stem Cell from Chronic Myeloid Leukemia patients

Liang WANG1,Xue-bin QU2,2,Chun-hua ZHAO1   

  1. 1. Center of Tissue Engineering, CAMS &PUMC
    2.
  • Received:2010-11-29 Revised:2011-01-19 Online:2011-04-05 Published:2011-04-08
  • Contact: Chun-hua ZHAO E-mail:zhaoch16@hotmail.com

摘要: 目的 研究慢性粒细胞白血病(CML)骨髓来源的肿瘤干细胞的免疫学特征,比较其与正常人来源的间充质干细胞(MSC)是否存在免疫功能的异常。方法 分离正常人和CML患者的骨髓中的MSC,MLR法检测其对T细胞增殖的影响,流式检测其对对T细胞周期、凋亡的作用情况。结果 CML和正常志愿者骨髓来源的MSC的细胞形态和表型没有差异,CML患者来源的MSC抑制T细胞增殖的能力和抑制T细胞停留在G0/G1期的作用(CML MSC组:74.5%±1.2%; BMSC组:94.0%±1.9%,p<0.05)均减弱,CML患者抑制T细胞凋亡的作用增强(CML MSC组:8.36%±1.31%;BMSC组:14.1%±0.65%,p<0.05)。结论 CML患者骨髓来源的MSC存在明显的免疫调节功能缺陷,如果使用CML患者自体的MSC移植治疗可能不是一种很好的选择,对于CML患者最好是选用异基因的MSC移植。

关键词: 慢性粒细胞白血病, 间充质干细胞, 免疫调节

Abstract: Objective To investigate that whether the immuno-modulatory capacity is intact in CML patients bone marrow derived MSCs and what are the differences compare with those from normal donors. Methods Isolate MSCs from CML patients and normal donors respectively and exam their differences on the T lymphocyte proliferation by MLR, on the T lymphocyte cycle and apoptosis using flow cytometry. Results The results show that there is no obvious difference on the immunophenotype between MSCs from CML and normal donors, respectively. However, for the MSCs from CML patients, the capacity of suppressing T cell proliferation as well as G0/G1 phase (CML MSC:74.5%±1.2%;BMSC:94.0%±1.9%, p<0.05) is weakened, while the capacity of suppressing T cell apoptosis is enhanced (CML MSC:8.36%±1.31%; BMSC:14.1%±0.65%, p<0.05). Conclusion We propose that MSCs from pathological environment might be abnormal and should not be used for autologous transplantation.

Key words: Chronic Myeloid Leukemia, Mesenchymal Stem Cell, immuno-modulatory

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